Novel gene members in the Pho regulon of Escherichia coli
Novel gene members in the Pho regulon of Escherichia coli
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DOI：
10.1111/j.1574-6968.2006.00440.x
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发表时间：
2006-11-01
影响因子：
2.1
通讯作者：
Lee, Sang Yup
中科院分区：
文献类型：
--
作者：
Baek, Jong Hwan;Lee, Sang Yup
比较野生型和phoB突变株在限磷条件下phoB和phoR基因表达最高的时间点上的转录组谱。在18个受PhoB转录调控因子控制的新推测基因中，通过序列分析鉴定出5个含有共识Pho box的基因。通过将这些基因的上游区域与无启动子的增强型绿色荧光蛋白基因融合，然后进行报告基因分析。结果发现，PhoB激活了amn （AMP核苷酶）、yibD（金属离子应激反应基因）和ytfK（假设蛋白）基因的表达。这些结果表明PhoB作为一个全局调节因子的其他作用。
The transcriptome profiles of the wild-type and the phoB mutant strains were compared at the time point showing the highest expression levels of the phoB and phoR genes under a P-limiting condition. Among the 18 new putative genes that were found to be under the control of the PhoB transcriptional regulator, five genes that contain the consensus Pho box were identified by sequence analysis. A reporter gene assay was carried out by fusing the upstream regions of these genes to the promoterless enhanced green fluorescent protein gene, followed by expression. It was found that the expressions of the amn (AMP nucleosidase), yibD (metal ion stress response gene) and ytfK (hypothetical protein) genes were activated by PhoB. These results indicate the additional roles of PhoB as a global regulator.