Effect of age on the response to in vitro ischemia and reperfusion of the rabbit bladder.
Effect of age on the response to in vitro ischemia and reperfusion of the rabbit bladder.
复制标题
年龄对兔膀胱体外缺血和再灌注反应的影响。
DOI：
10.1159/000098075
复制
发表时间：
2007
影响因子：
1.6
通讯作者：
Levin,RobertM
中科院分区：
文献类型：
--
作者：
Agartan,CananAldirmaz;Leggett,RobertE;Kogan,BarryA;Levin,RobertM
背景/目的：继发于出口梗阻的膀胱功能障碍是一种常见的疾病，见于儿童和成人。采用离体缺血/再灌注（I/R）模型，比较年轻和老年兔膀胱的收缩和生化反应。材料和方法：14只雄性新西兰白色兔分为两组，每组7只。第1组（幼龄）包括6-7周龄的家兔，第2组（老龄）包括2岁的家兔。将离体膀胱体条进行体外I/R，并测定对收缩性的影响。然后将条带冷冻并储存用于定量丙二醛（MDA）analysis.Results：年轻和老年兔膀胱对所有形式的刺激-场刺激（FS），卡巴胆碱和氯化钾（KCl）-的收缩反应是相等的。与组2相比，组1的32 Hz FS张力率显著更高。老年兔和青年兔对FS的I/R敏感性相同，但老年兔对卡巴胆碱和KCl刺激的I/R更敏感。青年和老年膀胱条的基础MDA浓度相似。缺血介导的MDA在膀胱条从年轻和老年兔显着增加，但MDA水平显着大于年轻比老年人。结论：虽然年轻兔膀胱的氧化损伤的水平更大，功能，老年兔膀胱更敏感I/R损伤。
Background/Aims:Urinary bladder dysfunction secondary to outlet obstruction is a common condition, seen in both children and adults. Using in vitro models of ischemia/reperfusion (I/R), we compared the contractile and biochemical responses of bladders isolated from young and old rabbits.Materials and Methods:Fourteen male New Zealand White rabbits were separated into two groups of 7. Group 1 (young) included rabbits 6–7 weeks old and group 2 (old) consisted of rabbits 2 years old. Isolated bladder body strips were subjected to in vitro I/R, and the effects on contractility were determined. The strips were then frozen and stored for quantitative malondialdehyde (MDA) analysis.Results:Contractile responses of young and old rabbit bladders to all forms of stimulation – field stimulation (FS), carbachol and potassium chloride (KCl) – were equal. The rate of tension to 32 Hz FS was significantly higher for group 1 in comparison with group 2. The old and young rabbits were equally sensitive to I/R in regard to FS, but the old rabbits were more sensitive to I/R in regard to carbachol and KCl stimulation. Basal MDA concentration of both young and aged bladder strips were similar. Ischemia mediated a significant increase in MDA in bladder strips from both young and old rabbits, but the MDA level was significantly greater for the young than for the old.Conclusion:Although the level of oxidative damage was greater in the young rabbit bladders, functionally, the old rabbit bladders were more sensitive to I/R damage.