Estimation of growth fraction with bromodeoxyuridine in human central nervous system tumors.
Estimation of growth fraction with bromodeoxyuridine in human central nervous system tumors.
复制标题
用溴脱氧尿苷估计人类中枢神经系统肿瘤的生长分数。
作者：
Y. Yoshii;Y. Maki;K. Tsuboi;Y. Tomono;K. Nakagawa;T. Hoshino
25例中枢神经系统肿瘤患者术前静脉滴注溴脱氧尿苷（BUdR）200 mg/m2，每8小时1次，连续3天。将切除的肿瘤标本固定在冷冻的70%乙醇中，包埋在石蜡中，并切成6微米切片。将每个切片与抗BUdR的单克隆抗体反应，并用免疫过氧化物酶染色以鉴定已掺入BUdR的细胞核。通过计算肿瘤存活区域中3 - 6个显微镜视野中BUdR阳性细胞核与肿瘤细胞总数的比率，估计每个肿瘤的生长分数。在7例病例中，通过连续计算机断层扫描测量肿瘤倍增时间，并尝试估计细胞周期时间。恶性胶质瘤的生长分数范围为9.1%至46.5%，低级别胶质瘤为2.0%至6.7%，转移性脑肿瘤为11.2%至43.2%，垂体腺瘤为0.8%至1.9%，听神经鞘瘤为3.9%至4.6%，脑膜瘤和小脑血管母细胞瘤为6.2%至8.2%。在大多数恶性胶质瘤和脑转移瘤中，估计的细胞周期时间为5至12天;然而，实际细胞周期时间应显著更短，因为计算中未考虑细胞损失。虽然生长分数似乎与每个肿瘤的生物学恶性程度相关，但肿瘤倍增时间并不反映生长潜力。不可预测的细胞损失可能在一定大小的肿瘤生长中起重要作用。因此，本研究中计算的细胞周期时间被大大高估，应谨慎解释。
Twenty-five patients with tumors of the central nervous system received bromodeoxyuridine (BUdR), 200 mg/sq m, by intravenous infusion every 8 hours for 3 days before surgery. Excised tumor specimens were fixed in chilled 70% ethanol, embedded in paraffin, and cut into 6-micron sections. Each section was reacted with monoclonal antibodies against BUdR and stained with immunoperoxidase to identify nuclei that had incorporated BUdR. The growth fraction of each tumor was estimated by calculating the ratio of BUdR-positive nuclei to the total number of tumor cells in three to six microscopic fields in viable areas of the tumor. In seven cases, the tumor doubling time was measured from the serial computerized tomography scans and an attempt was made to estimate the cell cycle time. The growth fractions ranged from 9.1% to 46.5% in malignant gliomas, 2.0% to 6.7% in low-grade gliomas, 11.2% to 43.2% in metastatic brain tumors, 0.8% to 1.9% in pituitary adenomas, 3.9% to 4.6% in acoustic neurinomas, and 6.2% to 8.2% in meningiomas and cerebellar hemangioblastomas. The estimated cell cycle time was 5 to 12 days in most malignant gliomas and brain metastases; however, the actual cell cycle time should be substantially shorter because cell loss was not considered in the calculation. Although the growth fraction appeared to correlate with the biological malignancy of each tumor, the tumor doubling time did not reflect growth potential. It is possible that unpredictable cell loss plays an important role in tumor growth at certain sizes. Therefore, the cell cycle times calculated in this study are considerably overestimated and should be interpreted with caution.
DOI：
10.1073/pnas.80.18.5573
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
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作者：
DOLBEARE, F;GRATZNER, H;GRAY, JW
通讯作者：
GRAY, JW