Glycoengineering and glycosite-specific labeling of serum IgGs from various species
Glycoengineering and glycosite-specific labeling of serum IgGs from various species
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不同物种血清 IgG 的糖工程和糖位点特异性标记
DOI：
10.1016/j.carres.2017.05.001
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发表时间：
2017-06-29
影响因子：
3.1
通讯作者：
Huang, Wei
中科院分区：
文献类型：
--
作者：
Guo, Weilai;Tang, Feng;Huang, Wei
内切S催化的免疫球蛋白G(Ig G)化学酶切糖工程是一种有效的改造Fc结构域N-糖异构体的方法，具有均一的合成糖链结构，以增强Fc受体介导的效应功能。以往的方法开发研究主要集中在人的或人源化的具有治疗潜力的免疫球蛋白。在这里，我们第一次报告了这种方法在包括兔、小鼠和山羊在内的其他物种的血清免疫球蛋白糖链重塑上的扩展应用。利用叠氮标记的非天然N-葡聚糖底物和连续的点击反应，实现了IGGS的糖位特异性荧光标记。本研究为免疫球蛋白的糖工程和Fc特异性标记提供了一条新的途径，该方法对抗原结合域的影响最小，适用于来自不同物种的数千种商品化抗体试剂，具有很大的应用潜力。(C)2017爱思唯尔有限公司。保留所有权利，
Chemoenzymatic glycoengineering of immunoglobulin G (IgG) catalyzed by Endo-S is a powerful approach to remodel the heterogeneous N-glycoforms of Fc domain with a homogeneous synthetic glycan structure for enhanced Fc receptor-mediated effector functions. The previous researches on the method development mainly focused on human or humanized IgGs with therapeutic potentials. Here, for the first time we report the extended application of this method on glycan-remodeling of serum IgGs from other species including rabbit, mouse, and goat. Harnessing an azido-tagged non-natural N-glycan substrate and successive click reaction, glycosite-specific fluorescent labeling of IgGs was enabled. This study provided a new avenue for glycoengineering and Fc-specific labeling of IgGs with minimized influence on antigen-binding domains, and this method was adaptive to thousands of commercial antibody reagents from various species with great application potentials. (C) 2017 Elsevier Ltd. All rights reserved,