An injectable and self-healing hydrogel for spatiotemporal protein release via fragmentation after passing through needles
An injectable and self-healing hydrogel for spatiotemporal protein release via fragmentation after passing through needles
复制标题
DOI：
10.1080/09205063.2017.1405573
复制
发表时间：
2018-01-01
影响因子：
3.6
通讯作者：
Ooya, Tooru
中科院分区：
文献类型：
--
作者：
Cho, Ik Sung;Ooya, Tooru
研究了通过混合乙二醇壳聚糖 (GC) 和氧化葡聚糖 (Odex) 配制的动态水凝胶与水凝胶破碎相结合的蛋白质控制释放。一系列可注射的动态水凝胶是由 GC 和 Odex 经过简单混合而得，无需添加化学交联剂。在生理pH值和温度下很容易发生凝胶化。考察了GC和Odex浓度对凝胶化时间、力学性能、含水量、体外降解的影响。 Odex/GC水凝胶在生理条件下表现出良好的自修复能力，并将动态席夫碱键保持在2 wt%以上。研究发现，模型蛋白（牛血清白蛋白）的释放动力学可以通过注射时改变针头尺寸来控制，这归因于即使在自愈状态下也能调节碎片水凝胶的表观尺寸和形状。因此，基于GC的可注射和动态水凝胶有望成为蛋白质递送系统和各种生物医学应用的有前景的平台。
A dynamic hydrogel formulated by mixing a glycol chitosan (GC) and an oxidized dextran (Odex) were studied for protein-controlled release in conjunction with the hydrogel fragmentation. A series of injectable dynamic hydrogels were derived from GC and Odex upon simple mixing without the addition of chemical crosslinking agents. The gelation readily took place at physiological pH and temperature. The influence of the concentration of GC and Odex on the gelation time, mechanical properties, water content, in vitro degradation were investigated. The Odex/GC hydrogels showed good self-healing ability under physiological conditions and kept the dynamic Schiff-base linkage at over 2 wt %. The release kinetics of a model protein (bovine serum albumin) was found to be controlled by changing the needle size upon injection, attributed to modulation of apparent size and shape of the fragmented hydrogels even in the self-healed state. Therefore, the GC-based injectable and dynamic hydrogels are expected to be a promising platform for protein delivery system and various biomedical applications.