Exosome-Mimetic Nanovesicles from Hepatocytes promote hepatocyte proliferation in vitro and liver regeneration in vivo.
Exosome-Mimetic Nanovesicles from Hepatocytes promote hepatocyte proliferation in vitro and liver regeneration in vivo.
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来自肝细胞的外泌体模拟纳米囊泡促进体外肝细胞增殖和体内肝再生
DOI：
10.1038/s41598-018-20505-y
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发表时间：
2018-02-06
影响因子：
4.6
通讯作者：
Jiang CP
中科院分区：
文献类型：
--
作者：
Wu JY;Ji AL;Wang ZX;Qiang GH;Qu Z;Wu JH;Jiang CP
肝脏在损伤或疾病导致的功能性质量损失后具有很大的再生能力。许多研究表明，原代肝细胞来源的外泌体可以在细胞间传递生物信息，促进肝脏的再生过程。然而，外泌体的产量非常有限。最近的研究表明，与外泌体相比，外泌体模拟纳米囊泡（NV）可以以几乎100倍的产率从细胞制备。因此，本研究探讨了来自原代肝细胞的外泌体模拟NV在肝脏再生中的治疗能力。通过连续挤压细胞通过聚碳酸酯膜制备外泌体模拟纳米粒子，这些纳米粒子的产量是外泌体的100倍以上。结果表明，NVs可通过显著提高受体细胞鞘氨醇激酶2的含量，促进肝细胞增殖和肝再生。据我们所知，这是第一次制备来自原代肝细胞的外泌体模拟NV，并且这些NV具有与来自原代肝细胞的外泌体相似的组分，并且在某些方面具有与外泌体相似的生物功能。受本研究启发的策略可能会导致用外泌体模拟NV取代外泌体以实现生物功能目的，包括用于组织修复和再生。
The liver has great regenerative capacity after functional mass loss caused by injury or disease. Many studies have shown that primary hepatocyte-derived exosomes, which can deliver biological information between cells, promote the regenerative process of the liver. However, the yield of exosomes is very limited. Recent studies have demonstrated that exosome-mimetic nanovesicles (NVs) can be prepared from cells with almost 100 times the production yield compared with exosomes. Thus, this study investigated the therapeutic capacity of exosome-mimetic NVs from primary hepatocytes in liver regeneration. Exosome-mimetic NVs were prepared by serial extrusions of cells through polycarbonate membranes, and the yield of these NVs was more than 100 times that of exosomes. The data indicated that the NVs could promote hepatocyte proliferation and liver regeneration by significantly enhancing the content of sphingosine kinase 2 in recipient cells. To the best of our knowledge, this is the first time that exosome-mimetic NVs from primary hepatocytes have been prepared, and these NVs have components similar to exosomes from primary hepatocytes and, in some respects, biofunctions similar to exosomes. Strategies inspired by this study may lead to substitution of exosomes with exosome-mimetic NVs for biofunctional purposes, including utilization in tissue repair and regeneration.
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影响因子：
3.7
作者：
Nojima H;Konishi T;Freeman CM;Schuster RM;Japtok L;Kleuser B;Edwards MJ;Gulbins E;Lentsch AB
通讯作者：
Lentsch AB
DOI：
10.1007/s11481-016-9724-3
发表时间：
2017-03
期刊：
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology
影响因子：
--
作者：
Joshi CR;Labhasetwar V;Ghorpade A
通讯作者：
Ghorpade A
将未成熟 DC 衍生的外泌体与供体抗原特异性 Treg 细胞相结合可在大鼠肝脏同种异体移植模型中诱导耐受性
影响因子：
4.6
作者：
Ma B;Yang JY;Song WJ;Ding R;Zhang ZC;Ji HC;Zhang X;Wang JL;Yang XS;Tao KS;Dou KF;Li X
通讯作者：
Li X
DOI：
10.4049/jimmunol.1203170
发表时间：
2013-04-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Deng ZB;Zhuang X;Ju S;Xiang X;Mu J;Liu Y;Jiang H;Zhang L;Mobley J;McClain C;Feng W;Grizzle W;Yan J;Miller D;Kronenberg M;Zhang HG
通讯作者：
Zhang HG
影响因子：
25.7
作者：
Nojima H;Freeman CM;Schuster RM;Japtok L;Kleuser B;Edwards MJ;Gulbins E;Lentsch AB
通讯作者：
Lentsch AB