Immunocytochemical demonstration of pituitary adenylate cyclase activating polypeptide (PACAP) in the porcine epiphyseal cartilage canals
Immunocytochemical demonstration of pituitary adenylate cyclase activating polypeptide (PACAP) in the porcine epiphyseal cartilage canals
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DOI：
10.1016/s0143-4179(97)90082-2
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发表时间：
1997-04-01
期刊：
影响因子：
2.9
通讯作者：
Hannibal, J
中科院分区：
文献类型：
--
作者：
StrangeVognsen, HH;Arnbjerg, J;Hannibal, J
腺苷酸环化酶激活多肽（PACAP）是血管活性肠多肽（VIP）/胰泌素/胰高血糖素家族的成员，已知是腺苷酸环化酶的强刺激剂。最近，PACAP已被证明可以刺激成骨细胞样细胞和小鼠跟骨中的cAMP。在本研究中，在新生猪和3-4周龄猪的软骨管中证实了PACAP免疫反应性（IR）。在有和没有骨化中心的股骨头和髌骨组织中，在支配血管的软骨管中发现了PACAP-IR神经纤维。分布模式不依赖于年龄或骨化中心的发生。共定位研究表明，降钙素基因相关肽（CGRP）和P物质（SP）的共定位程度较高，但与VIP的共定位很少。我们的研究结果支持CGRP，SP和VIP在骨组织中的早期研究结果，并将PACAP添加到骨组织中具有感觉和/或调节功能的神经肽组中。
Pituitary adenylate cyclase activating polypeptide (PACAP), a member of the vasoactive intestinal polypeptide (VIP)/secretin/glucagon family, is known to be a powerful stimulator of adenylate cyclase. Recently, PACAP has been shown to stimulate cAMP in osteoblast-like cells and mouse calvarian bones. In the present study, PACAP immunoreactivity (IR) was demonstrated in cartilage canals from newborn and 3-4-week-old pigs. In tissues from the femoral head and the patella with and without ossification centres, PACAP-IR nerve fibres were found in the cartilage canals innervating blood vessels. The pattern of distribution was not dependent on age or the occurrence of an ossification centre. Co-localization studies showed a high degree of co-localization with calcitonin gene-related peptide (CGRP) and substance P (SP) but little co-localization with VIP. Our findings support earlier findings of CGRP, SP and VIP in bone tissue and add PACAP to the group of neuropeptides with a sensory and/or modulatory function in bone tissue.