Laboratory setup for human in vitro fertilization.
Laboratory setup for human in vitro fertilization.
复制标题
人类体外受精实验室设置。
DOI：
10.1016/s0015-0282(16)47949-5
复制
发表时间：
1984
影响因子：
6.7
通讯作者：
M. M. Quigley
中科院分区：
文献类型：
--
作者：
P. Dandekar;M. M. Quigley
在人类IVF中使用的程序都不是独特的或技术上非常难以执行的。然而，在考虑所处理材料的性质时，所有实验室程序，设备和用品都必须是IVF的最佳选择。处理配子和胚胎的人员在处理人体组织之前，必须经过适当的培训，并具有处理哺乳动物配子和胚胎的经验。此外，技术人员必须接受适当的组织培养技术培训。除了技术人员的培训和经验之外，用于培养基制备的所有实验室设备和化学品必须具有最高质量和已知的一致性，并且使用小鼠胚胎的生物测定对培养条件进行定期检查。必须不断监测设备的运行情况，并尽可能建立备用系统，以尽量减少实验室事故导致胚胎丢失或损坏的可能性。对实验室程序的各个方面给予密切关注将使患者获得最佳的怀孕机会。
None of the procedures utilized in human IVF are unique or technically very difficult to perform. However, when considering the nature of the material being handled, it is essential that all laboratory procedures, equipment, and supplies are optimal for IVF. The individuals handling the gametes and embryos must be suitably trained and experienced in handling mammalian gametes and embryos before working with human tissues. In addition, the technologists must be trained in proper tissue culture techniques. Above and beyond the training and experience of the technologist, it is necessary that all laboratory equipment and chemicals for medium preparation be of the highest quality and known consistency, and that periodic checks of the culture conditions be performed using the bioassay of mouse embryos. The functioning of the equipment must be constantly monitored and backup systems be in place wherever possible in order to minimize the chances that laboratory accidents will result in loss or damage of the embryos. Meticulous attention to all aspects of the laboratory procedures will result in the patient's having the best possible chance to obtain a pregnancy.