Stable Isotope Techniques for the Assessment of Host and Microbiota Response During Gastrointestinal Dysfunction.
Stable Isotope Techniques for the Assessment of Host and Microbiota Response During Gastrointestinal Dysfunction.
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DOI：
10.1097/mpg.0000000000001373
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发表时间：
2017-01
影响因子：
2.9
通讯作者：
Morrison DJ
中科院分区：
文献类型：
--
作者：
Butler RN;Kosek M;Krebs NF;Loechl CU;Loy A;Owino VO;Zimmermann MB;Morrison DJ
国际原子能机构在维也纳召开了一次关于环境肠道功能障碍（EED）的技术会议（2015年10月28日至30日; https：//nucleus.iaea.org/HHW/Nutrition/EED_Technical_Meeting/index.html），汇集了EED、营养和稳定同位素技术领域的国际专家。稳定同位素标记技术的进步为我们更好地了解胃肠道功能障碍和微生物群在宿主健康中的作用开辟了新的可能性。在EED的背景下，对肠道功能障碍在宏量和微量营养素生物利用度和需求中可能发挥的作用以及营养状况和线性生长的后果知之甚少。稳定同位素标记技术已被用于评估肠粘膜损伤和屏障功能，碳水化合物消化和发酵，蛋白质衍生的氨基酸生物利用度和需求，微量营养素生物利用度和跟踪微生物-微生物和微生物-宿主在单细胞水平的相互作用。稳定同位素技术的非侵入性性质可能允许低危害，现场部署的肠道功能障碍测试，适用于所有年龄组。本综述的目的是评估国家的最先进的稳定同位素技术的使用，并提供一个角度来看，这些技术可以利用，以进一步了解肠道功能障碍的EED。
The International Atomic Energy Agency convened a technical meeting on environmental enteric dysfunction (EED) in Vienna (28th – 30th October 2015; https://nucleus.iaea.org/HHW/Nutrition/EED_Technical_Meeting/index.html) to bring together international experts in the fields of EED, nutrition and stable isotope technologies. Advances in stable isotope labelling techniques open up new possibilities to improve our understanding of gastrointestinal dysfunction and the role of the microbiota in host health. In the context of EED, little is known about the role gut dysfunction may play in macro- and micronutrient bioavailability and requirements and what the consequences may be for nutritional status and linear growth. Stable isotope labelling techniques have been used to assess intestinal mucosal injury and barrier function, carbohydrate digestion and fermentation, protein derived amino acid bioavailability and requirements, micronutrient bioavailability and to track microbe-microbe and microbe-host interactions at the single cell level. The non-invasive nature of stable isotope technologies potentially allows for low-hazard, field deployable tests of gut dysfunction that are applicable across all age-groups. The purpose of this review is to assess the state-of-the-art in the use of stable isotope technologies and to provide a perspective on where these technologies can be exploited to further our understanding of gut dysfunction in EED.