Immunological responsiveness of neonatal A/J mice to isotypic determinants of syngeneic IgE.
Immunological responsiveness of neonatal A/J mice to isotypic determinants of syngeneic IgE.
复制标题
新生 A/J 小鼠对同基因 IgE 同种型决定簇的免疫反应。
DOI：
10.1084/jem.168.2.713
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Nisonoff,A
中科院分区：
文献类型：
--
作者：
Haba,S;Nisonoff,A
我们以前已经证明，成年A/J小鼠产生高滴度的具有同型或独特型特异性的抗IgE，以KLH与同基因单抗IgE的结合反应。因此，小鼠体内存在能够合成抗IgE的B细胞。成年小鼠对CFA中未结合的IgE无反应，表明免疫耐受存在于T细胞水平。本研究表明，新生小鼠在CFA中产生抗IgE抗体，但这种能力在2-3周龄后丧失。反应性的丧失与血清中可检测到的IgE的出现密切相关，提示IgE可能诱导免疫耐受。新生小鼠产生的抗IgE抗体的亲和力落在成年小鼠的KLH-IgE获得的值范围内。新生小鼠在生理盐水中注射IgE可诱导对CFA中未结合的IgE的耐受。此外，过继移植成年小鼠的脾细胞可诱导出耐受状态。耐受的时间依赖性习得为研究耐受和自身免疫的机制提供了有用的模型。
We have previously shown that adult A/J mice produce high titers of anti-IgE with isotypic or idiotypic specificities in response to challenge with a conjugate of KLH with syngeneic monoclonal IgE. Thus, B cells that can synthesize anti-IgE are present in the mice. Adult mice are unresponsive to unconjugated IgE in CFA, suggesting that tolerance exists at the level of T cells. The present study shows that neonatal mice produce anti-IgE antibodies in response to unconjugated IgE in CFA, but that this capacity is lost after the age of 2-3 wk. The loss of responsiveness corresponds closely with the appearance of detectable IgE in serum, suggesting that the IgE may induce tolerance. The affinities of anti-IgE antibodies produced by neonatal mice fall in the range of values obtained with KLH-IgE in adult mice. Tolerance to unconjugated IgE in CFA can be induced in neonatal mice by administration of IgE in saline. In addition, the tolerant state can be induced by adoptive transfer of spleen cells from adult mice. The time-dependent acquisition of tolerance provides a useful model for studying mechanisms of tolerance and autoimmunity.