Tissue and species distribution and developmental variation of specific receptors for biologically active phorbol and ingenol esters.
Tissue and species distribution and developmental variation of specific receptors for biologically active phorbol and ingenol esters.
复制标题
生物活性佛波醇和巨大戟二萜醇酯的特定受体的组织和物种分布以及发育变化。
作者：
M. Shoyab;T. C. Warren;G. Todaro
本文报道了一种新的快速测定[~ 3 H]佛波醇-12，13-二丁酸酯（PDBu）与细胞或组织匀浆或亚细胞组分中受体结合的方法。PDBu结合位点广泛分布于小鼠组织中。大脑和脾脏含有异常高浓度的这些受体。PDBu受体存在于大脑的所有区域。脉络丛和垂体结合PDBu的量明显低于其他脑区。来自不同鼠种的大脑或脾脏结合几乎相同数量的PDBu，而来自胚胎小鼠的器官结合每一个小PDBu。结合随着年龄的增长而增加，并在约4周龄时达到最大值。不同物种的年轻和成熟的大脑都含有这些特异性结合位点。这些数据表明PDBu受体的结构或酶的作用。
A new and rapid binding assay for [3H]phorbol-12,13-dibutyrate (PDBu) binding to its receptors in cell or tissue homogenates or subcellular fractions is described. PDBu binding sites are widely distributed in mouse tissues. Brain and spleen contain exceptionally high concentrations of these receptors. PDBu receptors are present in all regions of the brain. The choroid plexus and pituitary bind markedly lower amounts of PDBu than other brain regions. Brains or spleens from different murine strains bind almost the same amounts of PDBu while those organs from embryonic mice bind every little PDBu. Binding increases with age and reaches a maximum at approximately 4 weeks of age. Young and mature brains from various species contain these specific binding sites. These data suggest a structural or enzymatic role for PDBu receptors.