Synthetic Macrocycle Nanopore for Potassium-Selective Transmembrane Transport
Synthetic Macrocycle Nanopore for Potassium-Selective Transmembrane Transport
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DOI：
10.1021/jacs.1c04910
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发表时间：
2021-08-17
影响因子：
15
通讯作者：
Feng, Jiandong
中科院分区：
文献类型：
--
作者：
Qiao, Dan;Joshi, Himanshu;Feng, Jiandong
合成纳米孔复制了生物膜通道的结构和功能，在膜过滤技术和生物分子传感中得到了应用。稳定的独立合成纳米孔已经由多种材料制成，包括多肽、核酸、合成聚合物和固态膜。然而，与生物纳米孔相比，为这种合成纳米孔提供原子定义的形状，包括故意放置每个化学基团，仍然是一个主要挑战。在这里，我们介绍一种化学合成大分子。延伸柱芳烃大环（EPM）。作为一种化学定义的跨膜纳米孔，具有选择性跨膜运输。我们的离子电流测量显示，EPM纳米孔稳定地插入脂质双层膜，并具有显著的阳离子类型选择性运输，钾离子的选择性高达钠离子的21倍。综上所述，直接化学合成为重新设计一类具有自定义传输功能的新型合成纳米孔提供了一条途径，这些纳米孔在其原子定义的化学结构中具有印记。
Reproducing the structure and function of biological membrane channels, synthetic nanopores have been developed for applications in membrane filtration technologies and biomolecular sensing. Stable stand-alone synthetic nanopores have been created from a variety of materials, including peptides, nucleic acids, synthetic polymers, and solid- state membranes. In contrast to biological nanopores, however, furnishing such synthetic nanopores with an atomically defined shape, including deliberate placement of each and every chemical group, remains a major challenge. Here, we introduce a chemosynthetic macromolecule.extended pillararene macrocycle (EPM).as a chemically defined transmembrane nanopore that exhibits selective transmembrane transport. Our ionic current measurements reveal stable insertion of individual EPM nanopores into a lipid bilayer membrane and remarkable cation type-selective transport, with up to a 21-fold selectivity for potassium over sodium ions. Taken together, direct chemical synthesis offers a path to de novo design of a new class of synthetic nanopores with custom transport functionality imprinted in their atomically defined chemical structure.