Functional alterations of swine peripheral blood mononuclear cells by methadone.
Functional alterations of swine peripheral blood mononuclear cells by methadone.
复制标题
美沙酮对猪外周血单核细胞功能的改变。
DOI：
10.1002/jlb.51.2.124
复制
发表时间：
1992
影响因子：
5.5
通讯作者：
Peterson,PK
中科院分区：
文献类型：
--
作者：
Molitor,TW;Murtaugh,MP;Click,RE;Gekker,G;Chao,C;Peterson,PK
合成阿片类药物美沙酮的体外暴露可以评估猪外周血单核细胞的假定免疫调节活性。呼吸爆发（杀菌活性的指标）在暴露 48 小时后被美沙酮以剂量依赖性方式抑制。阿片类拮抗剂纳洛酮可阻断这种抑制作用。巨噬细胞的另一种吞噬体-溶酶体融合功能因暴露于美沙酮而受损。原代淋巴细胞介导的功能自然杀伤细胞活性也受到影响。相反，巨噬细胞功能抗体介导的吞噬作用不受影响。由于美沙酮影响的功能对于宿主防御病原微生物至关重要，因此我们的研究结果表明阿片介导的免疫调节值得进一步研究。此外，我们的研究表明，猪可能为研究阿片介导的免疫细胞功能抑制提供理想的模型。
In vitro exposure of the synthetic opiate drug methadone allowed evaluation of putative immunomodulatory activities of swine peripheral blood mononuclear cells. Respiratory burst, an index of microbicidal activity, was suppressed by methadone in a dose‐dependent manner following exposure for 48 h. The suppression was blocked by the opiate antagonist naloxone. Another macrophage function phagosome‐lysosome fusion was impaired by exposure to methadone. A primary lymphocyte‐mediated function natural killer cell activity was also affected. In contrast, the macrophage function antibody‐mediated phagocytosis was not affected. Because the functions affected by methadone are critical to host defenses against pathogenic organisms, our findings suggest that opiate‐mediated immunomodulation merits further study. Moreover, our studies suggest that swine may provide an ideal model for the investigation of opiate‐mediated suppression of immune cell functions.