Peptide immobilisation on porous silicon surface for metal ions detection.
Peptide immobilisation on porous silicon surface for metal ions detection.
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DOI：
10.1186/1556-276x-6-412
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发表时间：
2011-06-06
影响因子：
--
通讯作者：
Gabouze NE
中科院分区：
文献类型：
--
作者：
Sam SS;Chazalviel JN;Gouget-Laemmel AC;Ozanam FF;Etcheberry AA;Gabouze NE
在这项工作中，一个甘氨酰-组氨酸-甘氨酰-组氨酸（GlyHisGlyHis）肽共价锚定到多孔硅PSi表面使用多步反应方案兼容的温和条件下，需要保留的探针活性。在第一步中，使用氢化硅烷化途径将烯烃前体接枝到氢化的PSi表面上，从而允许形成羧基封端的单层，其通过在肽偶联碳二亚胺N-乙基-N '-（3-二甲基氨基丙基）-碳二亚胺的存在下与N-羟基琥珀酰亚胺反应来活化，随后与肽的氨基接头反应以形成共价酰胺键。红外光谱（FT-IR）和X射线光电子能谱（XPS）用于研究功能化的不同步骤。利用多肽与金属离子形成稳定络合物的特性，通过肽修饰的基于PSI的生物传感器实现金属离子的识别。的电化学研究的GlyHisGlyHis修饰的PSi电极实现在铜离子的存在下。记录的循环伏安图显示了对应于Cu（II）/Cu（I）电对的准不可逆过程。测定了在多孔硅表面形成络合物的动力学因子（非均相速率常数和传递系数）和稳定常数。这些结果表明，在多孔硅上接枝的肽在开发简单，快速检测溶液中的金属离子的策略中的潜在作用。
In this work, a Glycyl-Histidyl-Glycyl-Histidine (GlyHisGlyHis) peptide is covalently anchored to the porous silicon PSi surface using a multi-step reaction scheme compatible with the mild conditions required for preserving the probe activity. In a first step, alkene precursors are grafted onto the hydrogenated PSi surface using the hydrosilylation route, allowing for the formation of a carboxyl-terminated monolayer which is activated by reaction with N-hydroxysuccinimide in the presence of a peptide-coupling carbodiimide N-ethyl-N'-(3-dimethylaminopropyl)-carbodiimide and subsequently reacted with the amino linker of the peptide to form a covalent amide bond. Infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy are used to investigate the different steps of functionalization. The property of peptides to form stable complexes with metal ions is exploited to achieve metal-ion recognition by the peptide-modified PSi-based biosensor. An electrochemical study of the GlyHisGlyHis-modified PSi electrode is achieved in the presence of copper ions. The recorded cyclic voltammograms show a quasi-irreversible process corresponding to the Cu(II)/Cu(I) couple. The kinetic factors (the heterogeneous rate constant and the transfer coefficient) and the stability constant of the complex formed on the porous silicon surface are determined. These results demonstrate the potential role of peptides grafted on porous silicon in developing strategies for simple and fast detection of metal ions in solution.