Visualizing priming of virus-specific CD8+ T cells by infected dendritic cells in vivo
Visualizing priming of virus-specific CD8+ T cells by infected dendritic cells in vivo
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DOI：
10.1038/ni762
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发表时间：
2002-03-01
影响因子：
30.5
通讯作者：
Yewdell, JW
中科院分区：
文献类型：
--
作者：
Norbury, CC;Malide, D;Yewdell, JW
合理设计能诱导CD8(+)T细胞反应的疫苗需要了解抗原提呈细胞(APC)的身份、提呈的位置和时间以及APC提呈的抗原的性质。在这里，我们针对一种由重组痘苗病毒编码的抗原解决这些问题。我们发现，在局部感染之后，痘苗病毒感染了引流淋巴结中的巨噬细胞和树突状细胞。然而，通过共聚焦显微镜直接观察切片的结节，只有树突状细胞递送抗原给初治的CD8(+)T细胞。接种后6h迅速出现，并随着感染细胞数量的增加而迅速下降。这些数据提供了病毒感染的APC在体内激活初始CD8(+)T细胞的直接证据。
The rational design of vaccines that elicit CD8(+) T cell responses requires knowledge of the identity of the antigen-presenting cell (APC), the location and time of presentation and the nature of the antigen presented by the APC. Here we address these questions for an antigen encoded by a recombinant vaccinia virus. We found that, following local infection, vaccinia virus infected macrophages and dendritic cells in draining lymph nodes. However, only the dendritic cells presented antigen to naive CD8(+) T cells, as determined by direct visualization of sectioned nodes by confocal microscopy. Presentation occurred as rapidly as 6 h after inoculation and quickly declined in parallel with the number of infected cells present in the nodes. These data provide direct evidence that virus-infected APCs prime naive CD8(+) T cells in vivo.