Development of a composite vascular scaffolding system that withstands physiological vascular conditions
Development of a composite vascular scaffolding system that withstands physiological vascular conditions
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DOI：
10.1016/j.biomaterials.2008.03.032
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发表时间：
2008-07-01
期刊：
影响因子：
14
通讯作者：
Yoo, James J.
中科院分区：
文献类型：
--
作者：
Lee, Sang Jin;Liu, Jie;Yoo, James J.
许多具有理想血管移植特性的支架已经被制造出来用于临床。然而，当这些支架暴露在包括高压和高流量在内的生理性血管环境中时，许多支架可能无法表现出一致的性能，并且由于意外的快速降解和低抗剪切应力，它们可能最终失效。需要开发一种更耐用的支架，能够承受这些条件，直到血管组织在体内成熟。本研究采用静电纺丝法制备了由聚c-己内酯（PCL）和胶原组成的血管支架。研究了复合支架的形态、生物力学和生物学性能。纤维直径约520 nm的PCL/胶原复合支架具有适当的抗拉强度（4.0 +/- 0.4 MPa）和足够的弹性（2.7 +/- 1.2 MPa）。复合支架的破裂压力为4912 +/- 155 mmHg，远高于单纯pcl支架（914 +/- 130 mmHg）和原生血管的破裂压力。将牛内皮细胞（bECs）和平滑肌细胞（bSMCs）分别植入复合支架后，在管腔内形成了一层bECs，在支架外表面形成了一层bSMCs。PCL/胶原复合支架具有生物相容性，具有长期抵抗高强度高压流动的生物力学特性，并提供支持血管细胞生长的良好环境。(C) 2008 Elsevier Ltd版权所有。
Numerous scaffolds that possess ideal characteristics for vascular grafts have been fabricated for clinical use. However, many of these scaffolds may not show consistent properties when they are exposed to physiologic vascular environments that include high pressure and flow, and they may eventually fail due to unexpected rapid degradation and low resistance to shear stress. There is a demand to develop a more durable scaffold that could withstand these conditions until vascular tissue matures in vivo. In this study, vascular scaffolds composed of poly(c-caprolactone) (PCL) and collagen were fabricated by electro-spinning. Morphological, biomechanical, and biological properties of these composite scaffolds were examined. The PCL/collagen composite scaffolds, with fiber diameters of approximately 520 nm, possessed appropriate tensile strength (4.0 +/- 0.4 MPa) and adequate elasticity (2.7 +/- 1.2 MPa). The burst pressure of the composite scaffolds was 4912 +/- 155 mmHg, which is much greater than that of the PCL-only scaffolds (914 +/- 130 mmHg) and native vessels. The composite scaffolds seeded with bovine endothelial cells (bECs) and smooth muscle cells (bSMCs) showed the formation of a confluent layer of bECs on the lumen and bSMCs on the outer surface of the scaffold. The PCL/collagen composite scaffolds are biocompatible, possess biomechanical properties that resist high degrees of pressurized flow over long term, and provide a favorable environment that supports the growth of vascular cells. (C) 2008 Elsevier Ltd. All rights reserved.