The NH2-terminal region of rabbit CYP2E1 is not essential for interaction with NADPH-cytochrome P450 reductase.
The NH2-terminal region of rabbit CYP2E1 is not essential for interaction with NADPH-cytochrome P450 reductase.
复制标题
兔 CYP2E1 的 NH2 末端区域对于与 NADPH-细胞色素 P450 还原酶的相互作用不是必需的。
DOI：
10.1006/bbrc.1994.2162
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发表时间：
1994
影响因子：
3.1
通讯作者：
Coon,MJ
中科院分区：
文献类型：
--
作者：
Voznesensky,AI;Schenkman,JB;Pernecky,SJ;Coon,MJ
Recently we reported that electrostatic forces interfere with the formation of the NADPH-cytochrome P450 reductase/cytochrome P450 electron transfer complex and suggested that this complex is formed by the attraction of the complementary hydrophobic patches (Voznesensky, A., and Schenkman,J.(1994)269J.Biol.Chem, 15724-1573). In this report we evaluate the role of the NH2-terminal hydrophobic region of CYP2E1 in the interaction with the reductase by comparing ionic strength dependence of the reduction of the full-length and truncated Δ3-29 CYP2E1. Increasing ionic strength stimulates reduction of both full-length and truncated CYP2E1. The neutralization of electrostatic interactions by increasing ionic strength revealed no impairment of the Δ3-29 CYP2E1 reduction compared to that of the full-length CYP2E1, indicating that the NH2-terminal region is not essential for the interaction of the cytochrome with the reductase.
