Antigenically distinct G glycoproteins of BRSV strains share a high degree of genetic homogeneity
Antigenically distinct G glycoproteins of BRSV strains share a high degree of genetic homogeneity
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DOI：
10.1006/viro.1997.8490
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发表时间：
1997-04-28
期刊：
影响因子：
3.7
通讯作者：
Taylor, G
中科院分区：
文献类型：
--
作者：
Furze, JM;Roberts, SR;Taylor, G
牛呼吸道合胞病毒（BRS）可根据单克隆抗体（mAb）对附着糖蛋白G的反应性分为不同的抗原亚型。此外，用表达特定亚组G蛋白的重组牛痘病毒接种的小牛的多克隆抗体应答也是亚组特异性的。为了研究BRS病毒G蛋白抗原异质性的遗传基础，对来自代表抗原亚群的6株BRS病毒株的G蛋白基因进行克隆、测序，并与原型亚群A株391-2进行比较。不同亚群菌株间的核酸差异仅为10%，氨基酸差异仅为15%。这些发现与人类RS病毒的情况形成鲜明对比，其中亚组之间的氨基酸同一性存在45%的差异。事实上，BRS病毒亚组之间的氨基酸差异程度与人类亚组内观察到的异质性水平相似。单克隆抗体与来自代表每个抗原亚组的G蛋白的富含半胱氨酸的区域（174-188）的肽的反应性分析表明，位置180、183和可能的184处的氨基酸在亚组区分中是重要的。总而言之，这些数据表明，尽管决定BRS病毒亚群抗原差异的遗传变异比在人类RS病毒亚群中观察到的更为有限，但存在的氨基酸差异对抗体识别具有深远的影响。(C)北京：科学出版社.
Bovine respiratory syncytial (BRS) virus can be divided into antigenic subgroups based on the reactivity of monoclonal antibodies (mAbs) to the attachment glycoprotein, G. Further, the polyclonal antibody response of calves vaccinated with recombinant vaccinia viruses expressing the G protein of a particular subgroup is also subgroup-specific. To investigate the genetic basis for the antigenic heterogeneity of the BRS virus G protein, the genes for the G protein from 6 BRS virus strains representative of the antigenic subgroups were cloned, sequenced, and compared with the prototype subgroup A strain, 391-2. There was only 10% nucleic acid difference and 15% amino acid difference between strains from different subgroups. These findings are in sharp contrast to the situation with human RS virus, where there is a 45% difference in amino acid identity between subgroups. In fact, the extent of amino acid difference between BRS virus subgroups is similar to the level of heterogeneity observed within human subgroups. Analysis of the reactivity of mAbs with peptides from the cysteine-rich region (174-188) of the G protein representing each antigenic subgroup indicated that amino acids at positions 180, 183, and possibly 184 are important in subgroup distinction. Taken together, these data suggest that although the genetic variation responsible for the antigenic differences determining subgroups among BRS viruses is more limited than that observed among human RS virus subgroups, the amino acid differences that exist have a profound effect upon antibody recognition. (C) 1997 Academic Press.