Preparation and characterization of novel polymeric microcapsules for live cell encapsulation and therapy
Preparation and characterization of novel polymeric microcapsules for live cell encapsulation and therapy
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DOI：
10.1385/cbb:47:1:159
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发表时间：
2007-01-01
影响因子：
2.6
通讯作者：
Prakash, Satya
中科院分区：
文献类型：
--
作者：
Chen, Hongmei;Wei Ouyang;Prakash, Satya
本文介绍了具有活细胞治疗应用潜力的新型京尼平交联型海藻酸盐微囊(GCAC)的制备和体外表征。该微胶囊体系由海藻酸盐核和共价交联壳聚糖膜组成，通过钙离子与海藻酸离子的电致胶凝作用形成微胶囊体系，然后通过聚电解质络合形成壳聚糖微胶囊，壳聚糖通过天然染料京尼平进行共价交联。结果表明，采用这种设计思想和三步法制备的球形GCAC微囊具有较高的膜强度、较低的包膜膨胀率和较好的通透性。以细菌植物乳杆菌80(PCBH1)(LP80)和哺乳动物细胞HepG2为模型细胞，评价了这种新型膜制剂对活细胞包埋的适用性。结果表明，包膜完整性和细菌细胞活力在包囊后6个月保持不变，表明该微胶囊制剂用于活细菌细胞包埋的可行性。并对其代谢活性进行了研究。结果表明，这种GCAC微囊在细胞治疗和细胞信号控制方面具有潜在的能力；然而，还需要进一步的研究。
This article describes the preparation and in vitro characterization of novel genipin cross-linked alginate-chi-tosan (GCAC) microcapsules that have potential for live cell therapy applications. This microcapsule system, consisting of an alginate core with a covalently cross-linked chitosan membrane, was formed via ionotropic gelation between calcium ions and alginate, followed by chitosan coating by polyelectrolyte complexation and covalent cross-linking of chitosan by naturally derived genipin. Results showed that, using this design concept and the three-step procedure, spherical GCAC microcapsules with improved membrane strength, suppressed capsular swelling, and suitable permeability can be prepared. The suitability of this novel membrane formulation for live cell encapsulation was evaluated, using bacterial Lactobacillus plantarum 80 (pCBH1) (LP80) and mammalian HepG2 as model cells. Results showed that capsular integrity and bacterial cell viability were sustained 6 mo postencapsulation, suggesting the feasibility of using this microcapsule formulation for live bacterial cell encapsulation. The metabolic activity of the encapsulated HepG2 was also investigated. Results suggested the potential capacity of this GCAC microcapsule in cell therapy and the control of cell signaling; however, further research is required.