Coupling of cell identity to signal response in yeast: interaction between the alpha 1 and STE12 proteins.
Coupling of cell identity to signal response in yeast: interaction between the alpha 1 and STE12 proteins.
复制标题
酵母中细胞身份与信号响应的耦合：α1 和 STE12 蛋白之间的相互作用。
DOI：
10.1101/gad.7.8.1584
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发表时间：
1993
影响因子：
10.5
通讯作者：
Fields,S
中科院分区：
文献类型：
--
作者：
Yuan,YO;Stroke,IL;Fields,S
在酿酒酵母中，STE 12蛋白介导响应于信息素的细胞类型特异性基因的转录诱导。STE 12在体外与存在于α-特异性基因的控制区中的信息素反应元件（PRE）结合。STE 12也是α特异性基因转录所必需的，但没有证据表明它直接与这些基因结合。相反，MAT α编码的蛋白α 1和MCM 1产物与负责α特异性和α因子诱导型表达的DNA元件结合。为了探索STE 12在α特异性基因的信息素诱导中的作用，我们从相关酵母乳酸克鲁维酵母中克隆了STE 12和MAT α 1同源物。酸克雷乳酸链球菌STE 12蛋白不与S.酿酒酵母α 1蛋白促进整个交配过程或诱导α特异性基因的转录。然而，引入K. lactis STE 12沿着K. lactis α 1确实恢复了交配，这表明STE 12和α 1之间的相互作用对于α特异性基因激活是重要的。我们还表明，细菌表达的STE 12和α 1能够在体外形成复合物。因此，我们证明了一个耦合在α细胞之间的蛋白质功能的细胞身份，α 1，与蛋白质响应信息素诱导的信号STE 12。
In Saccharomyces cerevisiae, the STE12 protein mediates transcriptional induction of cell type-specific genes in response to pheromones. STE12 binds in vitro to the pheromone response elements (PREs) present in the control region of a-specific genes. STE12 is also required for transcription of alpha-specific genes, but there is no evidence that it binds directly to these genes. Instead, the MAT alpha-encoded protein alpha 1 and the MCM1 product bind to the DNA element that is responsible for alpha-specific and a-factor-inducible expression. To explore the role of STE12 in the pheromone induction of alpha-specific genes, we cloned STE12 and MAT alpha 1 homologs from the related yeast Kluyveromyces lactis. The K. lactis STE12 protein did not cooperate with the S. cerevisiae alpha 1 protein to promote the overall mating process or the induction of transcription of an alpha-specific gene. However, introduction of both K. lactis STE12 along with K. lactis alpha 1 did restore mating, suggesting that an interaction between STE12 and alpha 1 is important for alpha-specific gene activation. We also show that bacterially expressed STE12 and alpha 1 are able to form a complex in vitro. Thus, we demonstrate a coupling in alpha cells between a protein functioning in cell identity, alpha 1, with a protein responsive to the pheromone-induced signal STE12.