Overexpression of IgLON cell adhesion molecules changesproliferation and cell size of cortical astrocytes
Overexpression of IgLON cell adhesion molecules changesproliferation and cell size of cortical astrocytes
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IgLON细胞粘附分子的过度表达改变皮质星形胶质细胞的增殖和细胞大小
DOI：
10.1002/cbf.2813
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发表时间：
2013
影响因子：
3.6
通讯作者：
Seiji Miyata
中科院分区：
文献类型：
--
作者：
Chiaki Sugimoto;Shoko Morita;Seiji Miyata
IgLON家族是免疫球蛋白超家族细胞粘附分子的一个亚组，由边缘系统相关蛋白（LAMP）、阿片样物质结合细胞粘附分子（OBCAM）、神经三肽（Ntm）和Kilon组成。在本研究中，我们研究了LAMP，OBCAM，Ntm和Kilon的过表达对体外1型星形胶质细胞增殖和细胞大小的影响。溴脱氧尿苷免疫细胞化学定量分析显示，OBCAM的表达对星形胶质细胞增殖的抑制作用强于LAMP、Ntm和Kilon。与对照组相比，OBCAM过表达增加了星形胶质细胞的细胞大小。与对照组相比，FGF-2处理对OBCAM转染的星形胶质细胞具有更大的增殖作用。这些结果表明，与其他IgLON蛋白相比，OBCAM是控制星形胶质细胞增殖和细胞大小的更有效的调节剂，可能通过FGF-2受体介导的途径。版权所有© 2012约翰威利父子有限公司.
IgLON family is a subgroup of the immunoglobulin superfamily cell adhesion molecules and composed of limbic system‐associated protein (LAMP), opioid binding cell adhesion molecule (OBCAM), neurotrimin (Ntm) and Kilon. In the present study, we investigated the overexpression of LAMP, OBCAM, Ntm and Kilon on the proliferation and cell size of type‐1 astrocytesin vitro. Quantitative analysis using bromodeoxyuridine immunocytochemistry revealed that the expression of OBCAM had greater inhibitory effect on astrocytic proliferation as compared with LAMP, Ntm and Kilon ones. OBCAM overexpression increased the cell size of astrocytes as compared with the control. The treatment of FGF‐2 had greater proliferative effect on OBCAM‐transfected astrocytes as compared with the control. These results suggest that OBCAM is more potent regulator for controlling the proliferation and cell size of astrocytes as compared with other IgLON proteins possibly through FGF‐2 receptor‐mediated pathway. Copyright © 2012 John Wiley & Sons, Ltd.