Interactions between tubulointerstitial nephritis antigen and laminin or type IV collagen.
Interactions between tubulointerstitial nephritis antigen and laminin or type IV collagen.
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肾小管间质性肾炎抗原与层粘连蛋白或 IV 型胶原之间的相互作用。
DOI：
10.1159/000422978
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发表时间：
1994
影响因子：
--
通讯作者：
Thull,J
中科院分区：
文献类型：
--
作者：
Charonis,A;Kalfa,T;Butkowski,R;Thull,J
材料和方法如前所述[1，2]，通过胶原酶消化和离子交换和凝胶渗透色谱法从兔肾皮质基底膜中提取和纯化TIN-ag。将纯化的TIN-抗原储存在-20 ℃。层粘连蛋白和IV型胶原蛋白根据先前描述的方案[3，4]从皮下生长在LAA小鼠中的EHS肿瘤中提取。层粘连蛋白储存在液氮中，IV型胶原储存在冰上。使用前，通过离心清除所有大分子的大聚集体。
Materials and MethodsTIN-ag was extracted and purified from rabbit kidney cortex basement membranes by collagenase digestion and ion-exchange and gel permeation chromatography as previously described [1, 2]. The purified TIN-ag was stored at-20 C. Laminin and type IV collagen were extracted from EHS tumor grown subcutaneously in lathyritic mice according to protocols previously described [3, 4]. Laminin was stored in liquid nitrogen and type IV collagen was stored on ice. Before use, all macromolecules were cleared of large aggregates by centrifugation.