Septin-Mediated Plant Cell Invasion by the Rice Blast Fungus, Magnaporthe oryzae
Septin-Mediated Plant Cell Invasion by the Rice Blast Fungus, Magnaporthe oryzae
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DOI：
10.1126/science.1222934
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发表时间：
2012-06-22
期刊：
影响因子：
56.9
通讯作者：
Talbot, Nicholas J.
中科院分区：
文献类型：
--
作者：
Dagdas, Yasin F.;Yoshino, Kae;Talbot, Nicholas J.
为了引起稻瘟病，稻瘟病菌会产生一个被称为附着胞的加压圆顶状细胞，它会物理性地破坏叶片角质层，从而进入植物组织。在这里，我们报告说，一个环形的F-肌动蛋白网络组装在附着胞通过四个septin鸟苷三磷酸酶，这是一个动态的，异源寡聚环。隔膜支架F-肌动蛋白，通过ezrin-radixin-膜突蛋白Tea 1，和磷脂酰肌醇在附着胞质膜的相互作用。隔蛋白环以Cdc 42-和Chm 1-依赖的方式组装，并形成扩散屏障，以在穿透点定位反向bin-两栖physin-RVS-结构域蛋白Rvs 167和Wiskott-Aldrich综合征蛋白Las 17。因此，隔膜提供了皮质刚性和膜曲率，这是刚性穿透钉突出以破坏叶片表面所必需的。
To cause rice blast disease, the fungus Magnaporthe oryzae develops a pressurized dome-shaped cell called an appressorium, which physically ruptures the leaf cuticle to gain entry to plant tissue. Here, we report that a toroidal F-actin network assembles in the appressorium by means of four septin guanosine triphosphatases, which polymerize into a dynamic, hetero-oligomeric ring. Septins scaffold F-actin, via the ezrin-radixin-moesin protein Tea1, and phosphatidylinositide interactions at the appressorium plasma membrane. The septin ring assembles in a Cdc42- and Chm1-dependent manner and forms a diffusion barrier to localize the inverse-bin-amphiphysin-RVS-domain protein Rvs167 and the Wiskott-Aldrich syndrome protein Las17 at the point of penetration. Septins thereby provide the cortical rigidity and membrane curvature necessary for protrusion of a rigid penetration peg to breach the leaf surface.