CYTOPLASMIC GENETICS OF MAMMALIAN-CELLS - CONDITIONAL SENSITIVITY TO MITOCHONDRIAL INHIBITORS AND ISOLATION OF NEW MUTANT PHENOTYPES
CYTOPLASMIC GENETICS OF MAMMALIAN-CELLS - CONDITIONAL SENSITIVITY TO MITOCHONDRIAL INHIBITORS AND ISOLATION OF NEW MUTANT PHENOTYPES
复制标题
DOI：
10.1007/bf01542645
复制
发表时间：
1979-01-01
期刊：
影响因子：
--
通讯作者：
SAGER, R
中科院分区：
文献类型：
--
作者：
HOWELL, N;SAGER, R
作为碳源的葡萄糖和丙酮酸是胞质传递的氯霉素耐药（CAP-R）突变的表型表达、CAP-R突变体的恢复以及在寡霉素或抗霉素存在下的持续生长所必需的。当线粒体呼吸减少时，葡萄糖可以提供额外的能量，当克雷布斯循环被抑制时，丙酮酸可以提供中间体。对丙酮酸的需求完全由嘌呤的外源性来源满足，部分由α满足。-酮戊二酸酯或嘧啶源。获得两种影响线粒体功能的突变，寡霉素耐药性和氯霉素耐药性的丙酮酸非依赖性表达。两者都是细胞质传递的，为线粒体生物发生的遗传分析提供了新的标记。
Glucose, as a carbon source, and pyruvate are required for the phenotypic expression of cytoplasmically transmitted chloramphenicol-resistance (CAP-R) mutations, recovery of CAP-R mutants, and continuous growth in the presence of oligomycin or antimycin. Glucose may supply additional energy when mitochondrial respiration is diminished and pyruvate may provide intermediates when the Krebs cycle is inhibited. The requirement for pyruvate is fully satisfied by an exogenous source of purines, and partially by .alpha.-ketoglutarate or a pyrimidine source. Two types of mutations affecting mitochondrial function, oligomycin resistance and pyruvate-independent expression of chloramphenicol resistance, were obtained. Both are cytoplasmically transmitted and provide new markers for a genetic analysis of mitochondrial biogenesis.