Characterization of Fertile Homozygous Genotypes from Anther Culture in Apple
Characterization of Fertile Homozygous Genotypes from Anther Culture in Apple
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苹果花药培养可育纯合基因型的特征
DOI：
10.21273/jashs.134.6.641
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发表时间：
2009
影响因子：
1.9
通讯作者：
S. Komori
中科院分区：
文献类型：
--
作者：
Hatsuhiko Okada;Yoshitaka Ohashi;Mamoru Sato;Hideyuki Matsuno;Toshiya Yamamoto;Hoytaek Kim;Tatsuro Tukuni;S. Komori
附加索引词。雄激素发生、双单倍体、流式细胞术、苹果·国产、形态学、SSR 分析、S 等位基因摘要。加倍单倍体可以提高苹果(Malus·domestica Borkh.)育种和遗传研究的效率。 'Senshu'苹果通过体外花药培养获得17个纯合基因型。流式细胞术分析表明，花药衍生植株的倍性水平是二倍体。简单序列重复（SSR）分析确定了花药衍生植株的起源和纯合状态。 S-RNase 聚合酶链式反应 (PCR) 消化分析的结果强化了纯合状态。研究了双单倍体的形态特征和繁殖潜力。特别有趣的是，来自花药培养的双单倍体之一具有可育的花粉粒（体外发芽率为20.6％～33.0％），并且‘Prima’苹果与双单倍体之间的杂交获得了多个后代。这些幼苗的杂交性通过SSR分析得到证实。此外，双单倍体的雌配子表现出轻微的生育能力。因此，这种双单倍体对于育种和遗传研究将具有重要价值。
ADDITIONAL INDEX WORDS. androgenesis, doubled haploids, flow cytometry, Malus ·domestica, morphology, SSR analysis, S-allele ABSTRACT. Doubled haploids can improve the efficiency of breeding and genetic study in apple (Malus ·domestica Borkh.). Seventeen homozygous genotypes were obtained by in vitro anther culture from 'Senshu' apple. Flow cytometry analysis revealed that the ploidy level of the anther-derived plantlets was diploid. Simple sequence repeat (SSR) analysis determined the origin and homozygous status of the anther-derived plantlets. The results of S-RNase polymerase chain reaction (PCR)-digestion analysis reinforced the homozygous state. The morphological charac- teristics and reproductive potential of the doubled haploids were investigated. It is especially interesting that one of the doubled haploids derived from anther culture had fertile pollen grains (rate of in vitro germination was 20.6%- 33.0%), and several progenies were obtained from the cross between 'Prima' apple and the doubled haploid. The hybridism of these seedlings was confirmed by SSR analysis. Furthermore, the female gametes of the doubled haploid showed slight fertility. Thus, this doubled haploid will be valuable for breeding and genetic studies.
可育单倍体柚子（Citrus grandis Osbeck）的形态特征。
DOI：
--
发表时间：
2005
期刊：
J.Amer.Soc Hort Sci 130・1
影响因子：
--
作者：
Yahata;M.;S.Harusaki;K.Takami;P.Toolapong;H.Kunitake.T.Yabuya(他2名)
通讯作者：
H.Kunitake.T.Yabuya(他2名)
通过 S-RNase 分析和 SSR 标记对日本苹果品种进行分子表征。
DOI：
--
发表时间：
2005
期刊：
J Amer Soc Hort Sd 130
影响因子：
--
作者：
Kitahara;K.;et. al.
通讯作者：
et. al.