Gut lavage IgG and interleukin 1 receptor antagonist:interleukin 1 beta ratio as markers of intestinal inflammation in children with inflammatory bowel disease.
Gut lavage IgG and interleukin 1 receptor antagonist:interleukin 1 beta ratio as markers of intestinal inflammation in children with inflammatory bowel disease.
复制标题
肠道灌洗 IgG 和白细胞介素 1 受体拮抗剂：白细胞介素 1 β 比率作为炎症性肠病儿童肠道炎症的标志物。
作者：
R. Troncone;N. Caputo;A. Campanozzi;M. Cucciardi;V. Esposito;R. Russo;B. de Vizia;L. Greco;S. Cucchiara
背景：目前，全肠灌洗被用作大肠放射学或内镜检查前的准备工作。目的：通过测定肠灌洗液中IgG和白细胞介素1 β (IL-1 β)的含量，验证肠灌洗技术对粘膜炎症的评价。患者：16名患有克罗恩病（CD）的儿童，14名患有溃疡性结肠炎（UC）的儿童，22名年龄匹配的对照组。方法：采用等渗、不可吸收的聚乙二醇基灌洗液口服或鼻胃管灌洗。收集清液，过滤后用蛋白酶抑制剂处理。采用夹心酶联免疫吸附法（ELISA）检测IgG、IL-1 β和IL-1受体拮抗剂（IL-1-ra）含量。结果：UC和CD患者的IgG和IL-1 β水平显著高于对照组（p < 0.001）。与疾病活动度评分呈正相关（p < 0.05）。与对照组相比，UC和CD患者IL-1-ra水平无显著差异，但UC和CD患者IL-1-ra:IL-1 β比值显著（p < 0.01）降低，且与灌洗液IgG水平呈负相关（p < 0.001）。结论：肠道灌洗液IgG和IL-1 β水平及IL-1-ra:IL-1 β比值可作为炎性肠病患儿活动性和非活动性疾病的客观鉴别指标。
BACKGROUND: Whole gut lavage is currently used as preparation before radiological or endoscopic examination of the large bowel. AIM: To validate the gut lavage technique for the assessment of mucosal inflammation, by measuring intestinal IgG and interleukin 1 beta (IL-1 beta) in the fluid obtained. PATIENTS: Sixteen children with Crohn's disease (CD), 14 with ulcerative colitis (UC), and 22 age matched controls. METHODS: Isotonic, non-absorbable polyethylene glycol based lavage solution was given orally or by nasogastric tube. Clear fluid was collected, filtered, and treated with protease inhibitors. IgG, IL-1 beta and IL-1-receptor antagonist (IL-1-ra) were measured by sandwich enzyme linked immunosorbent assay (ELISA). RESULTS: In patients with UC and CD, IgG and IL-1 beta levels were significantly (p < 0.001) higher than in controls. A positive correlation (p < 0.05) was found with disease activity scores. IL-1-ra levels were not significantly different in UC and CD, when compared with controls, but the IL-1-ra:IL-1 beta ratio was significantly (p < 0.01) lower in patients with UC and CD, and negatively (p < 0.001) correlated with IgG levels in lavage fluid. CONCLUSIONS: Gut lavage fluid IgG and IL-1 beta levels and IL-1-ra:IL-1 beta ratio may provide objective discrimination between active and inactive disease in children with inflammatory bowel disease.
聚乙二醇电解质溶液用于顽固性大便失禁儿童的肠道清除。
DOI：
10.1001/archpedi.1988.02150030114035
发表时间：
1988
期刊：
American journal of diseases of children (1960)
影响因子：
--
作者：
Ingebo,KB;Heyman,MB
通讯作者：
Heyman,MB
影响因子：
15.9
作者：
COMINELLI, F;NAST, CC;DINARELLO, CA
通讯作者：
DINARELLO, CA