Sequence and promoter regulation of the PCK1 gene encoding phosphoenolpyruvate carboxykinase of the fungal pathogen Candida albicans
Sequence and promoter regulation of the PCK1 gene encoding phosphoenolpyruvate carboxykinase of the fungal pathogen Candida albicans
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DOI：
10.1016/s0378-1119(97)00069-3
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发表时间：
1997-06-19
期刊：
影响因子：
3.5
通讯作者：
Ernst, JF
中科院分区：
文献类型：
--
作者：
Leuker, CE;Sonneborn, A;Ernst, JF
从白色念珠菌（Candida albicans）中分离并克隆了编码PEP羧激酶（Pck 1）的PCK 1基因。推导的Pck 1蛋白与其他物种的ATP依赖的Pck 1蛋白有很高的同源性，特别是与酿酒酵母的Pck 1（70%同源性），但与GTP依赖的Pck 1蛋白没有同源性。PCK 1的转录水平被葡萄糖有效地抑制，并在非同源碳源上被去抑制（诱导）。PCK 1调控发生在转录水平上，如通过PCK 1启动子与LAC 4报告基因的融合所证明的，产生>100的去阻遏/阻遏表达比率。C.白色念珠菌和S. cerevisiae进行鉴定。PCK 1启动子可用于有效调控表达，从而检测C.白色念珠菌(C)1997年Elsevier Science B.V.
The PCK1 gene encoding PEP carboxykinase (Pck1) of the fungal pathogen Candida albicans was isolated and sequenced. The deduced Pck1 protein has high homology to ATP-dependent Pck1 proteins in other species, especially to Pck1 of Saccharomyces cerevisiae (70% homology), but not to GTP-dependent Pck1 proteins. PCK1 transcript levels were efficiently repressed by glucose and derepressed (induced) on gluconeogenetic carbon sources. PCK1 regulation occurs on the level of transcription, as demonstrated by a fusion of the PCK1 promoter to the LAC4 reporter gene, yielding derepressed/repressed expression ratios of >100. Homologous sequences in the PCK1 promoters of C. albicans and S. cerevisiae were identified. The PCK1 promoter may be useful to efficiently regulate expression and thereby test the function of genes in C. albicans. (C) 1997 Elsevier Science B.V.