The δ Subunit of RNA Polymerase Is Required for Rapid Changes in Gene Expression and Competitive Fitness of the Cell
The δ Subunit of RNA Polymerase Is Required for Rapid Changes in Gene Expression and Competitive Fitness of the Cell
复制标题
DOI：
10.1128/jb.00188-13
复制
发表时间：
2013-06-01
影响因子：
3.2
通讯作者：
Krasny, Libor
中科院分区：
文献类型：
--
作者：
Rabatinova, Alzbeta;Sanderova, Hana;Krasny, Libor
RNA聚合酶（RNAP）是一种被广泛研究的多亚基酶，它是DNA转录成RNA所必需的，然而RNAP的δ亚基仍然是一个谜，其生理作用尚未完全阐明。在这里，我们确定了一个新的，迄今未被识别的功能δ枯草芽孢杆菌。我们证明了delta通过起始核苷三磷酸（[iNTP]）的浓度影响RNAP的调节，这是一种重要的机制，对于基因表达响应环境变化的快速变化至关重要。因此，我们证明了在不断变化的环境中面对竞争菌株时，δ对细胞存活至关重要。因此，尽管delta本身不是必需的，但它对细胞在自然界中快速适应和生存的能力至关重要。最后，我们表明，其他两个蛋白质，GreA和Yde B，以前牵连到影响其他生物体中的RNAP的调节[iNTP]，没有这种功能在B。枯草杆菌。
RNA polymerase (RNAP) is an extensively studied multisubunit enzyme required for transcription of DNA into RNA, yet the delta subunit of RNAP remains an enigmatic protein whose physiological roles have not been fully elucidated. Here, we identify a novel, so far unrecognized function of delta from Bacillus subtilis. We demonstrate that delta affects the regulation of RNAP by the concentration of the initiating nucleoside triphosphate ([iNTP]), an important mechanism crucial for rapid changes in gene expression in response to environmental changes. Consequently, we demonstrate that delta is essential for cell survival when facing a competing strain in a changing environment. Hence, although delta is not essential per se, it is vital for the cell's ability to rapidly adapt and survive in nature. Finally, we show that two other proteins, GreA and YdeB, previously implicated to affect regulation of RNAP by [iNTP] in other organisms, do not have this function in B. subtilis.