Activation and significance of vacuolar H+-ATPase in Saccharomyces cerevisiae adaptation and resistance to the herbicide 2,4-dichlorophenoxyacetic acid
Activation and significance of vacuolar H+-ATPase in Saccharomyces cerevisiae adaptation and resistance to the herbicide 2,4-dichlorophenoxyacetic acid
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DOI：
10.1016/j.bbrc.2003.11.072
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发表时间：
2003-12-26
影响因子：
3.1
通讯作者：
Sá-Correia, I
中科院分区：
文献类型：
--
作者：
Fernandes, AR;Durao, PJ;Sá-Correia, I
本文报道了2，4-二氯苯氧乙酸(2，4-D)对酿酒酵母液泡膜H~+-ATPase活性的刺激作用。这种体内的激活(高达5倍)基本上发生在适应期，在除草剂胁迫下细胞分裂之前，伴随着质膜H+-ATPase(PM-ATPase)的显著激活(高达30倍)以及细胞内和液泡pH值的下降，这表明激活可能是由酸化触发的。VMA1和其他V-ATPasc亚基编码基因的单一缺失导致了更长的适应期和在2，4-D胁迫下的缓慢生长。结果表明，在2，4-D适应过程中，需要一个功能性的V-ATPase来更快、更有效地抵消跨越液泡膜的生理W梯度的消散。(C)2003 Elsevier Inc.保留所有权利。
The stimulation of the activity of the H+-ATPase present in the vacuolar membrane (V-ATPase) of Saccharomyces cerevisiae is here described in response to a moderate stress induced by 2,4-dichlorophenoxyacetic acid (2,4-D). This in vivo activation (up to 5-fold) took place essentially during the adaptation period, preceding cell division under herbicide stress, in coordination with a marked activation of plasma membrane H+-ATPase (PM-ATPase) (up to 30-fold) and the decrease of intracellular and vacuolar pH values, suggesting that activation may be triggered by acidification. Single deletion of VMA1 and genes encoding other V-ATPasc subunits led to a more extended period of adaptation and to slower growth under 2,4-D stress. Results suggest that a functional V-ATPase is required to counteract, more rapidly and efficiently, the dissipation of the physiological W-gradient across vacuolar membrane registered during 2,4-D adaptation. (C) 2003 Elsevier Inc. All rights reserved.