Control of retention by molecular interactions in reversed-phase chromatography
Control of retention by molecular interactions in reversed-phase chromatography
复制标题
反相色谱中通过分子相互作用控制保留
DOI：
10.1016/0021-9673(93)80797-c
复制
发表时间：
1993
影响因子：
4.1
通讯作者：
R. Scott
中科院分区：
文献类型：
--
作者：
R. Scott
描述了不同类型的反相合成中使用的程序，以及产品的物理和化学性质。不同的反相的表面的性质进行了讨论，并在与水接触时发生的变化解释的基础上的分散特性的粘结相和水的高极性。的低聚物相的固有的稳定性被认为是和溶剂的吸附从水性溶剂混合物的表面上的反相描述，包括吸附等温线的形式。溶质分子可以与反相表面相互作用的不同方式进行了讨论，并使用简单的液-液分配系统检查在移动的相发生的相互作用。结果表明，在溶剂混合物中，溶质与给定溶剂相互作用的概率与溶剂的体积分数成正比。然后考虑溶剂混合物的组分之间发生缔合的条件，例如使用甲醇的含水溶剂混合物。由此产生的三元混合物被认为增加了保留机制的复杂性。最后，给出了一个通用方程，该方程描述了溶质在每对固定相和移动的相组分之间的分配系数以及可用于溶质相互作用的各相组分的分数方面的保留体积。
The procedures used in the synthesis of the different types of reversed phases are described, together with the physical and chemical properties of the products. The nature of the surface of the different reversed phases is discussed and the changes that take place when in contact with water explained on the basis of the dispersive character of the bonded phase and the high polarity of the water. The innate stability of the oligomeric phases is considered and the adsorption of solvent from aqueous solvent mixtures onto the surface of a reversed phase described, including the form of the adsorption isotherm. The different ways a solute molecule can interact with the reversed-phase surface is discussed and the interactions that take place in the mobile phase examined using simple liquid—liquid distribution systems. It is shown that the probability of solute interaction with a given solvent in a solvent mixture is proportional to the volume fraction of the solvent. Conditions are then considered where association between the components of the solvent mixture occurs using, as an example, aqueous solvent mixtures of methanol. The resulting ternary mixture is seen to add to the complexity of the retention mechanism. Finally, a general equation is given that describes the retention volume of a solute in terms of the distribution coefficient of the solute between each pair of stationary phase and mobile phase components and the fraction of each phase component available for solute interaction.