The biological activity of cytokinin derivatives in the soybean callus bioassay

The biological activity of cytokinin derivatives in the soybean callus bioassay
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DOI:
10.1007/bf00024957
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发表时间:
1991-05
影响因子:
4.2
通讯作者:
J. Staden;F. E. Drewes
J. Staden;F. E. Drewes
中科院分区:
生物学3区
文献类型:
--
作者:
J. Staden;F. E. Drewes

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研究了28种天然和人工合成的细胞分裂素(包括细胞分裂素核苷酸)对大豆子叶愈伤组织生长的影响。一般来说,核苷和核苷酸比它们各自的游离碱基给出稍好的响应。然而,反应的差异并不显着,并且有一种明显的可能性,即这三种类型的细胞分裂素之间的快速相互转化发生在组织内。Z和ZR的O-糖苷活性最高。3、7和9位的糖基化降低活性。在BA衍生物的情况下,N-葡萄糖苷的活性顺序为3G > 9 G> 7 G。由于异戊烯基衍生物的活性很低,因此很难用大豆愈伤组织生物测定法检测。
The effect of 28 natural and synthetic cytokinins, including cytokinin nucleotides, the growth of soybean cotyledonary callus was investigated. Generally the nucleosides and nucleotides gave a slightly better response than their respective free bases. The differences in response were, however, not significant and there is a distinct possibility that rapid interconversions between these three types of cytokinin occur within the tissue. The O-glucosides of Z and ZR were the most active. Glucosylation in the 3, 7 and 9 positions reduced activity. In the case of BA-derivatives the order of activity of the N-glucosides was 3G > 9G > 7G. Sinceiso-pentenyl derivatives had little activity they may be very difficult to detect using the soybean callus bioassay.