Involvement of V-ATPases in the digestion of soft connective tissue collagen
Involvement of V-ATPases in the digestion of soft connective tissue collagen
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DOI：
10.1006/bbrc.1998.9357
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发表时间：
1998-10-20
影响因子：
3.1
通讯作者：
Everts, V
中科院分区：
文献类型：
--
作者：
Creemers, LB;Jansen, IDC;Everts, V
在软结缔组织外植体（骨膜）中研究了空泡H+-ATP酶（V-ATP酶）对胶原降解的贡献。免疫定位显示整个骨膜的细胞染色微弱到强烈。V-ATP酶抑制剂巴弗洛霉素A（1）和福利霉素在24和48小时后分别使总胶原降解减少40%和50%。V-ATP酶参与细胞内胶原的降解，通过抑制泵活性后成纤维细胞中吞噬胶原的量减少来证明。降解的抑制不是由于明胶酶A的活性降低，明胶酶A是一种以前发现的介导胶原降解的酶，通过组织和条件培养基的酶谱分析进行评估。巴弗洛霉素A（1）甚至诱导两种组分中明胶酶A和B水平的增加。总之，酸化的V-ATP酶可能是一个重要的机制，在细胞外和细胞内的胶原降解软结缔组织。(C)北京：科学出版社.
The contribution of vacuolar H+-ATPases (V-ATPases) to collagen degradation was investigated in soft connective tissue explants (periosteum). Immunolocalisation showed faint to intense staining of cells throughout the periosteum. The V-ATPase inhibitors, bafilomycin A(1) and folimycin, decreased overall collagen degradation by 40 and 50% after 24 and 48 h, respectively. The participation of V-ATPases in intracellular degradation of collagen was demonstrated by the decrease of the amount of phagocytosed collagen in fibroblasts upon inhibition of pump activity. The inhibition of degradation was not due to a reduction in activity of gelatinase A, an enzyme previously found to mediate collagen degradation, as assessed by zymographic analysis of tissue and conditioned medium. Bafilomycin A(1) even induced an increase of gelatinase A and B levels in both fractions. In conclusion, acidification by V-ATPases may represent an important mechanism in extracellular and intracellular collagen degradation in soft connective tissue. (C) 1998 Academic Press.