Phenotypic and genetic characterization of tomato mutants provides new insights into leaf development and its relationship to agronomic traits
Phenotypic and genetic characterization of tomato mutants provides new insights into leaf development and its relationship to agronomic traits
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DOI：
10.1186/s12870-019-1735-9
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发表时间：
2019-04-15
影响因子：
5.3
通讯作者：
Moreno, Vicente
中科院分区：
文献类型：
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作者：
Jaquez-Gutierrez, Marybel;Atares, Alejandro;Moreno, Vicente
背景：叶子形态改变的番茄突变体通常在温室中鉴定，这需要相当长的时间和空间，并且只能在足够的时间内进行。为了获得更快但同样可靠的检查方法，我们解决了 971T-DNA 系的体外筛选问题。在幼苗和芽衍生的无菌植物中体外评估叶子发育。在温室中对新突变体进行了表征，以建立体外和体内叶片形态之间的关系，并揭示叶片发育和农艺性状之间可能存在的联系，这是一个有待发现的有前景的领域。 结果：在体外筛选番茄 T-DNA 品系后，在温室中评估了叶片形态改变的假定突变体。两种条件下结果的比较表明了一般表型对应关系，表明体外培养是寻找叶片发育中改变的突变体的可靠系统。除了提供均质条件外，体外筛选的主要优点还在于节省大量时间和空间。表型和 nptII 基因表达之间关联的研究表明两个品系存在共分离 (P > 99%)。增强子陷阱的使用还允许通过报告基因表达分析来识别功能获得突变体。这些研究表明，其他三个突变体中发生改变的基因带有 T-DNA 标记。新的突变体可能改变了油菜素类固醇的合成或感知，突变决定了多种多效性效应，影响了器官曲率的线条，并且发现了第一个具有螺旋生长的番茄突变体。结果还揭示了叶片发育和农艺性状之间新的可能联系，例如腋生分枝、花脱落、果实发育和裂果。此外，我们发现突变体 2635-MM 中标记的基因编码甾醇 3-β-葡萄糖基转移酶。表达分析表明叶片发育异常可能是由于该基因功能缺失所致。结论：体外培养是鉴定叶片形态改变的番茄突变体的快速、有效和可靠的工具。新突变体的体内表征揭示了叶片发育和一些农艺性状之间的新联系。此外，还报道了编码甾醇3-β-葡萄糖基转移酶的基因在番茄叶发育中的可能意义。
Background: Tomato mutants altered in leaf morphology are usually identified in the greenhouse, which demands considerable time and space and can only be performed in adequate periods. For a faster but equally reliable scrutiny method we addressed the screening in vitro of 971T-DNA lines. Leaf development was evaluated in vitro in seedlings and shoot-derived axenic plants. New mutants were characterized in the greenhouse to establish the relationship between in vitro and in vivo leaf morphology, and to shed light on possible links between leaf development and agronomic traits, a promising field in which much remains to be discovered.Results: Following the screening in vitro of tomato T-DNA lines, putative mutants altered in leaf morphology were evaluated in the greenhouse. The comparison of results in both conditions indicated a general phenotypic correspondence, showing that in vitro culture is a reliable system for finding mutants altered in leaf development. Apart from providing homogeneous conditions, the main advantage of screening in vitro lies in the enormous time and space saving. Studies on the association between phenotype and nptII gene expression showed co-segregation in two lines (P > 99%). The use of an enhancer trap also allowed identifying gain-of-function mutants through reporter expression analysis. These studies suggested that genes altered in three other mutants were T-DNA tagged. New mutants putatively altered in brassinosteroid synthesis or perception, mutations determining multiple pleiotropic effects, lines affected in organ curvature, and the first tomato mutant with helical growth were discovered. Results also revealed new possible links between leaf development and agronomic traits, such as axillary branching, flower abscission, fruit development and fruit cracking. Furthermore, we found that the gene tagged in mutant 2635-MM encodes a Sterol 3-beta-glucosyltransferase. Expression analysis suggested that abnormal leaf development might be due to the lack-off-function of this gene.Conclusion: In vitro culture is a quick, efficient and reliable tool for identifying tomato mutants altered in leaf morphology. The characterization of new mutants in vivo revealed new links between leaf development and some agronomic traits. Moreover, the possible implication of a gene encoding a Sterol 3-beta-glucosyltransferase in tomato leaf development is reported.