Plasma phospholipid metabolic profiling and biomarkers of rats following radiation exposure based on liquid chromatography-mass spectrometry technique
Plasma phospholipid metabolic profiling and biomarkers of rats following radiation exposure based on liquid chromatography-mass spectrometry technique
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DOI：
10.1002/bmc.1226
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发表时间：
2009-10-01
影响因子：
1.8
通讯作者：
Nie, Jihua
中科院分区：
文献类型：
--
作者：
Wang, Chang;Yang, Jun;Nie, Jihua
脂质组学是代谢组学的一个重要领域，它利用新的分析方法来研究脂质分子的广泛类别、脂质代谢的变化和脂质介导的信号传导过程。本文采用高效液相色谱-质谱联用技术（HPLC-MS）和多元统计分析相结合的方法，研究了γ射线照射后大鼠血浆中磷脂代谢的变化及可能的生物标志物。采用正交偏最小二乘-潜在结构判别分析（OPLS-DA）方法，寻找辐射暴露大鼠血浆磷脂的潜在生物标志物。根据相应的串联质谱结果，鉴定潜在的生物标志物。γ射线照射后，磷脂酰乙醇胺和磷脂酰丝氨酸明显增加，磷脂酰胆碱、鞘磷脂和溶血磷脂酰胆碱也有同样的趋势，但增加不明显。结果提示，受照大鼠存在磷脂代谢异常，这可能是一种评价辐射暴露的替代方法。这些生物标志物可能参与辐射诱导的细胞凋亡途径，是发现新的辐射防护药物和辐射增敏剂的有希望的靶点。版权所有（C）2009约翰威利父子有限公司
Lipidomics, a prominent area of metabolomics, utilizes novel analytical methodologies to study the extensive classes of lipid molecules, changes in lipid metabolism and lipid-mediated signaling processes. In this paper, the phospholipid metabolic profiles changes and potential biomarker identification in the rats plasma after gamma-irradiation exposure were investigated by coupling high performance liquid chromatography-mass spectrometry technology to multivariate statistical analysis. Orthogonal partial least-squares to latent structures discriminate analysis (OPLS-DA) was used to find the potential plasma phospholipids biomarkers of rats for radiation exposure. According to the corresponding tandem mass spectrometric results, potential biomarkers were identified. After exposure to gamma-rays, phosphatidylethanolamine and phosphatidylserine showed a marked increase, and phosphatidylcholine, sphingomyelin and lysophosphatidylcholine followed the same trend, although their rise was not significant. The results suggested that radiated rats had a phospholipid metabolic abnormality, which could be an alternative way to assess the radiaton exposure. The biomarkers may involve a radiation-induced apoptosis pathway and represent a promising target for discovery new radioprotective drugs and radiosensitizers. Copyright (C) 2009 John Wiley & Sons, Ltd.