Characterization of a new calcium-binding protein abundant in amniotic fluid, CAAF2, which is produced by fetal epidermal keratinocytes during embryogenesis.
Characterization of a new calcium-binding protein abundant in amniotic fluid, CAAF2, which is produced by fetal epidermal keratinocytes during embryogenesis.
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羊水中丰富的新型钙结合蛋白 CAAF2 的表征，该蛋白在胚胎发生过程中由胎儿表皮角质形成细胞产生。
DOI：
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
K. Yamaguchi
中科院分区：
文献类型：
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作者：
J. Hitomi;K. Maruyama;Y. Kikuchi;K. Nagasaki;K. Yamaguchi
利用~（45）Ca ~（2+）覆盖技术，我们在牛羊水中发现了两种丰富的钙结合蛋白（CaBP），分别命名为CAAF 1（calcium-binding protein in amniotic fluid-1）和CAAF 2。CAAF 1是一种由胎儿表皮角质形成细胞、鳞状上皮细胞和多形核白细胞产生的新的S100蛋白。在这里，我们报告的一级结构和牛CAAF 2的组织分布。CAAF 2是一个新分离的蛋白质，具有S100蛋白特有的两个EF手基序，与CAAF 1的同源性为29.7%。CAAF 2与银屑病表皮中表达上调的S100蛋白--人银屑病素有63.4%的同源性。北方印迹分析表明，CAAF 2是独特的，因为它只在表皮中表达。我们的研究结果表明，羊水中的大多数CaBP由两种不同的S100蛋白，CAAF 1和CAAF 2，这是由胎儿表皮角质形成细胞产生的。
By using a 45Ca2+ overlay technique, we found two calcium-binding proteins (CaBPs) abundant in bovine amniotic fluid, and named them CAAF1 (calcium-binding protein in amniotic fluid-1) and CAAF2. CAAF1 was identified as a novel S100 protein produced by fetal epidermal keratinocytes, squamous epithelial cells and polymorphonuclear leukocytes. Here, we report the primary structure and the tissue distribution of bovine CAAF2. CAAF2 is a newly isolated protein with the two EF hand motifs peculiar to S100 proteins, and 29.7% homology to CAAF1. CAAF2 is 63.4% homologous to human psoriasin, a S100 protein up-regulated in psoriatic epidermis. Northern blot analysis demonstrates that CAAF2 is unique in that it is exclusively expressed in the epidermis. Our results suggest that the majority of CaBPs in amniotic fluid consist of two distinct S100 proteins, CAAF1 and CAAF2, which are produced by fetal epidermal keratinocytes.