A Simple Approach to Gas Chromatographic Microanalysis of Alcohols in Blood and Urine by a Direct-Injection Technique
A Simple Approach to Gas Chromatographic Microanalysis of Alcohols in Blood and Urine by a Direct-Injection Technique
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采用直接进样技术对血液和尿液中的酒精进行气相色谱微量分析的简单方法
DOI：
10.1093/jat/2.6.257
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发表时间：
1978
影响因子：
2.5
通讯作者：
J. E. Manno
中科院分区：
文献类型：
--
作者：
B. Manno;J. E. Manno
建立了直接进样超微量气相色谱同时测定血液和尿液中乙醇、甲醇、异丙醇和丙酮的方法。所有的全血、血清、血浆或尿液和一种内标物--正丙醇等份混合在一起。然后将一微米的混合物注入岛津GC-4BM气相色谱仪，该色谱仪配有8个火焰电离检测器。玻璃柱，直径3毫米。X 2-m长，在801100 Carbopak C(Supalco，Inc.，Bellelento，PA#SP-1827.)上填充0.2%CarBowax 1500。色谱柱安装了S柱前玻璃插件，填充了一个松散包装的8ct8玻璃棉塞子，作为对血液最终尿样中非挥发性蛋白质物质的捕捉器。色谱仪的最佳操作条件为：柱温120~进样温度100℃，载气流量20ml/mln，氢气和气压设定为最佳的8氢火焰条件，洗脱物的相对保留时间为：乙醇0.51，甲醇0.37，丙酮0.65，异丙醇0.78。本文还介绍了一种简便的微量血样酒精分析方法，为临床或法医毒理学中经常遇到的酒精的单次或同时超微量分析提供了一种快速、重复性好的方法。
An ultra-micro method utilizing direct sample Injection Is described for the simultaneous Identification and quantification of ethanol, methanol, Isopropanol, and acetone In blood and urine by gas chromatography. AIIquots of whole blood, serum, plasma, or urine and an Internal standard, 1-propanol, are mixed together In equal parts. One microllter of the mixture Is then Injected Into a Shimadzu GC-4BM gas chromatograph equipped with 8 flame Ionization detector. The glass column, 3-mm I.d. x 2-m long, Is packed with 0.2% Carbowax 1500 on 801100 Carbopak C (Supalco, Inc., Bellelento, PA #SP-1827). The column Is fitted with s precolumn glass insert filled with a loosely packed sllanlzed glass wool plug that 8ct8 as a trap for the nonvolatile protein material of the blood end urine specimens. The optimal operating condltions for the gas chromatograph are: column temperature 120~ Injector temperature 100"C; carrier gas flow 20 ml/mln; hydrogen and sir pressure set st optimal conditions for 8 hydrogen flame. The relative retention times of the eluted compounds to the Internal standard, l-propsnol, are: ethanol 0.51; methanol 0.37; acetone 0.65; and Isopropanol 0.78. A simple micro method Is also described for the celiaclion and storage of blood for alcohol determination. The techniques provide a rapid, reproducible procedure for the individual or simultaneous ultra-micro analysis of alcohols cominertly encountered in clinical or forensic toxicology.