Proliferation characteristics of coxsackievirus A10 in mice and immune protection ability of experimental inactivated vaccine
Proliferation characteristics of coxsackievirus A10 in mice and immune protection ability of experimental inactivated vaccine
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DOI：
10.1016/j.biopha.2021.112212
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发表时间：
2021-09-27
影响因子：
7.5
通讯作者：
Xie, Zhongping
中科院分区：
文献类型：
--
作者：
Gao, Weijie;Yue, Lei;Xie, Zhongping
柯萨奇病毒A10（Coxsackievirus A10，CVA 10）是我国手足口病的主要病原体。然而，目前还没有针对CVA 10的特异性药物和疫苗，这种病毒在体内的发病机制和影响尚不清楚。我们研究了临床分离的CVA 10病毒株（CVA 10 -25）通过不同感染途径对乳鼠的影响。我们用不同剂量的病毒感染乳鼠，用相同剂量的病毒感染不同年龄的小鼠，观察病毒在小鼠组织中的动态分布和增殖情况。并对感染后的病理特征进行了分析。制备甲酰胺灭活的实验疫苗，免疫5周龄BALB/c雌性小鼠3次，并检测新生乳鼠是否存在母传抗体。脑内给药后各器官的病毒载量均高于腹腔给药后;经口给药途径未引起小鼠发病。小鼠感染后瘫痪和死亡与年龄有关。感染后骨骼肌、心脏和肺出现组织病理学改变。我们建立了2日龄BALB/c乳鼠脑内感染模型，以研究CVA 10的发病机制和病理变化。母体传播的抗体保护小鼠免受病毒的侵害。本研究为CVA 10相关致病机制及疫苗研究提供了实验依据。
Coxsackievirus A10 (CVA10) is the main pathogen of hand, foot, and mouth disease in China. However, there are no CVA10-specific drugs and vaccines, and the pathogenesis and effects of this virus in the body are unknown. We investigated the effect of a clinically isolated CVA10 virus strain (CVA10-25) to investigate its effect in suckling mice through different infection routes. We observed the dynamic distribution and proliferation of the virus in mouse tissues by infecting suckling mice with different doses of the virus and mice of different ages with the same dose of the virus. We also analysed the pathological characteristics after infection. A formaldehyde-inactivated experimental vaccine was prepared to immunise 5-week-old BALB/c female mice three times, and newborn suckling mice were tested for the presence of maternally transmitted antibodies. The viral load in each organ after intracerebral administration was higher than that after intraperitoneal administration; the peroral administration route did not cause disease in mice. Mouse paralysis and death after infection were related to age. The skeletal muscles, heart, and lung showed histopathological changes after infection. We established a 2-day -old BALB/c suckling mouse model that could be infected intracranially to study the pathogenesis and pathology of CVA10. Maternally transmitted antibodies protected the mice against the virus. This study provides a refer-ence for CVA10-related pathogenesis and vaccine research.