Fluorescence and absorption contrast mechanisms for biomedical optical imaging using frequency-domain techniques
Fluorescence and absorption contrast mechanisms for biomedical optical imaging using frequency-domain techniques
复制标题
DOI：
10.1111/j.1751-1097.1997.tb03138.x
复制
发表时间：
1997-07-01
影响因子：
3.3
通讯作者：
Hutchinson, CL
中科院分区：
文献类型：
--
作者：
SevickMuraca, EM;Lopez, G;Hutchinson, CL
光学成像或检测位于组织深处的患病组织体积的能力取决于由局部光学特性的差异提供的对比度。在这份报告中，我们表明，外源的对比度提供的荧光化合物是上级提供的非荧光，光吸收化合物时，采用时间依赖性的测量，此外，我们表明，诱导的对比度不仅受到荧光剂优先摄取到感兴趣的患病组织体积中的调节，而且还受到特定组织中的荧光光学性质和荧光动力学的调节。组织体积。使用组织模型研究，我们实验性地证明了近红外吸收和荧光染料如吲哚菁绿色可以从在组织外围进行的荧光测量提供患病组织体积的检测，此时在患病组织中存在相对于周围正常组织中的完美的、100倍和10倍的分区，普通激光染料的实验结果表明，对比度也介导的量子产率和寿命参数，可能取决于局部组织环境。
The ability to optically image or detect diseased tissue volumes located deep within tissues depends upon the degree of contrast provided by differences in local optical properties. In this report, we show that the exogenous contrast offered by fluorescent compounds is superior to that provided by nonfluorescing, light-absorbing compounds when time-dependent measurements are employed, In addition, we show that the induced contrast is not only moderated by the preferential uptake of fluorescent agents into diseased tissue volumes of interest but also by the fluorescent optical properties and the fluorescence dynamics in the specific tissue volume, Using tissue phantom studies, we demonstrated experimentally that near-infrared-absorbing and fluorescent dyes such as indocyanine green can provide detection of diseased tissue volumes from fluorescence measurements made at the periphery off tissue when there is perfect, 100-fold and 10-fold partitioning in diseased tissues over that in surrounding normal tissues, Experimental results of common laser dyes show the contrast is also mediated by the quantum yield and lifetime parameters that may be dependent upon the local tissue environment.