Cloning and characterization of a member of the MST subfamily of Ste20-like kinases
Cloning and characterization of a member of the MST subfamily of Ste20-like kinases
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DOI：
10.1016/0378-1119(95)00653-2
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发表时间：
1995-12-29
期刊：
影响因子：
3.5
通讯作者：
Chernoff, J
中科院分区：
文献类型：
--
作者：
Creasy, CL;Chernoff, J
我们已经确定了第二个人类同源的酵母Ste 20蛋白激酶家族，我们指定MST 2。MST 2与先前鉴定的MST 1蛋白激酶最相似（78%同一性，88%相似性）。北方分析表明，MST 2 mRNA在成人肾脏、骨骼和胎盘组织中以高水平表达，而在成人心脏、肺、肝和脑组织中以非常低的水平表达。体外激酶测定表明，MST 2可以磷酸化外源性底物，以及本身，和磷酸氨基酸分析表明，它是一个丝氨酸/苏氨酸蛋白激酶。MST 2的鉴定表明可能存在Ste 20样蛋白激酶的亚家族，并且MST 1和MST 2可能定义这些亚家族之一。
We have identified a second human homolog of the yeast Ste20 protein kinase family, which we designate MST2. MST2 is most similar to the previously identified MST1 protein kinase (78% identity, 88% similarity). Northern analysis indicates that MST2 mRNA is expressed at high levels in adult kidney, skeletal and placental tissues and at very low levels in adult heart, lung, liver and brain tissues. An in vitro kinase assay indicates that MST2 can phosphorylate an exogenous substrate, as well as itself, and phospho-amino-acid analysis indicates that it is a serine/threonine protein kinase. The identification of MST2 suggests that there may be subfamilies of Ste20-like protein kinases and that MST1 and MST2 may define one of these subfamilies.