Isolation of a novel gene, moc2, encoding a putative RNA helicase as a suppressor of sterile strains in Schizosaccharomyces pombe
Isolation of a novel gene, moc2, encoding a putative RNA helicase as a suppressor of sterile strains in Schizosaccharomyces pombe
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DOI：
10.1016/s0167-4781(99)00071-8
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发表时间：
1999-07-07
期刊：
影响因子：
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通讯作者：
Kawamukai, M
中科院分区：
文献类型：
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作者：
Kawamukai, M
从裂殖酵母中分离到一个编码RNA解旋酶的新基因moc2，该基因抑制了两个不同突变株的不育性，其中一个突变株的cAMP水平升高，另一个突变株的RAS功能减弱，这是显性负RAS2异位表达的结果。MOC2与酿酒酵母的RNA解旋酶DED1和小鼠的PL10高度同源(同源性分别为58%和50%)。Moc2基因的破坏表明moc2是细胞生长所必需的。Moc2基因似乎对性分化和细胞生长都有作用。(C)1999 Elsevier Science B.V.保留所有权利。
A novel gene designated moc2, which encodes a putative RNA helicase, was isolated from Schizosaccharomyces pombe on the basis of its suppression of the sterility of two different mutant strains, one of which had elevated levels of cAMP and the other deregulated Ras functioning as a result of an ectopic expression of dominant negative RAS2. Moc2 is highly homologous to the RNA helicase DED1 of Saccharomyces cerevisiae (58% identity) and PL10 of mouse (50% identity). Disruption of the moc2 gene indicated that moc2 is essential for cell growth. The moc2 gene seems to have roles in both sexual differentiation and cell growth. (C) 1999 Elsevier Science B.V. All rights reserved.