Mimicry Technology: Suppressing Small RNA Activity in Plants
Mimicry Technology: Suppressing Small RNA Activity in Plants
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DOI：
10.1007/978-1-61779-083-6_10
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发表时间：
2011-01-01
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影响因子：
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通讯作者：
Manavella, Pablo Andres
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文献类型：
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作者：
Rubio-Somoza, Ignacio;Manavella, Pablo Andres
小RNA的抑制是对其在植物中的特殊作用进行完整分子表征的主要困难之一。利用对microRNA （miRNA）活性调控新生物发生后层的最新见解，有可能克服上述限制（Nat Genet 39:1033-1037,2007）。我们设计了IPSI非编码RNA，使其与给定的miRNA家族具有互补序列，从而导致RISC复合物的特异性隔离。MIMIC技术允许组成性释放miRNA家族的所有潜在靶点，以及组织特异性和诱导性抑制其活性。
Small RNA suppression constitutes one of the major difficulties for a full molecular characterization of their specific roles in plants. Taking advantage of the latest insights into the new post-biogenesis layer of regulation in microRNA (miRNA) activity, it is possible to overcome the above-mentioned limitation (Nat Genet 39:1033-1037,2007). We engineered the IPSI non-coding RNA to bear a complementary sequence to a given miRNA family, resulting in specific sequestration of RISC complexes. MIMIC technology allows for the constitutive release of all of the potential targets of a miRNA family as well as tissue-specific and inducible suppression of its activity.