THE USE OF A CHARGE-COUPLED DEVICE FOR QUANTITATIVE OPTICAL MICROSCOPY OF BIOLOGICAL STRUCTURES
THE USE OF A CHARGE-COUPLED DEVICE FOR QUANTITATIVE OPTICAL MICROSCOPY OF BIOLOGICAL STRUCTURES
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DOI：
10.1126/science.3116667
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发表时间：
1987-10-02
期刊：
影响因子：
56.9
通讯作者：
AGARD, DA
中科院分区：
文献类型：
--
作者：
HIRAOKA, Y;SEDAT, JW;AGARD, DA
描述了电荷耦合器件 (CCD) 的特性及其在光学显微镜高分辨率生物结构分析中的应用。 CCD具有高分辨率、高灵敏度、宽动态范围、光度精度和几何稳定性，可以提供高质量的数据，使得对二维和三维显微图像进行定量分析成为可能。例如，落射荧光显微镜的三维成像特性已通过 CCD 进行定量测定。对显微镜成像特性的描述以及 CCD 提供的高质量图像数据允许使用复杂的计算图像处理方法，从而大大提高生物结构可获得的有效分辨率。图像处理技术揭示了果蝇胚胎二倍体染色体的二维和三维精细亚结构。同样的方法可以扩展到像酵母染色体这样小的结构或结构细胞生物学中的其他问题。
The properties of a charge-coupled device (CCD) and its application to the high-resolution analysis of biological structures by optical microscopy are described. The CCD, with its high resolution, high sensitivity, wide dynamic range, photometric accuracy, and geometric stability, can provide data of such high quality that quantitative analysis on two- and three-dimensional microscopic images is possible. For example, the three-dimensional imaging properties of an epifluorescence microscope have been quantitatively determined with the CCD. This description of the imaging properties of the microscope, and the high-quality image data provided by the CCD, allow sophisticated computational image processing methods to be used that greatly improve the effective resolution obtainable for biological structures. Image processing techniques revealed fine substructures inDrosophilaembryonic diploid chromosomes in two and three dimensions. The same approach can be extended to structures as small as yeast chromosomes or to other problems in structural cell biology.