Treatment Paradigms for Retinal and Macular Diseases Using 3-D Retina Cultures Derived From Human Reporter Pluripotent Stem Cell Lines.
Treatment Paradigms for Retinal and Macular Diseases Using 3-D Retina Cultures Derived From Human Reporter Pluripotent Stem Cell Lines.
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DOI：
10.1167/iovs.15-17639
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发表时间：
2016-04-01
影响因子：
4.4
通讯作者：
Swaroop A
中科院分区：
文献类型：
--
作者：
Kaewkhaw R;Swaroop M;Homma K;Nakamura J;Brooks M;Kaya KD;Chaitankar V;Michael S;Tawa G;Zou J;Rao M;Zheng W;Cogliati T;Swaroop A
我们讨论了使用多能干细胞系携带由视网膜启动子驱动的荧光报告程序在培养中获得三维(3-D)视网膜，以及如何利用该系统来阐明人类视网膜生物学，在培养皿中创建疾病模型，并设计针对视网膜和黄斑变性的靶向药物筛选。此外，我们意识到干细胞研究是劳动密集型的，需要广泛的资源。为了通过共享资源加速科学发现并避免重复工作，我们建议成立视网膜干细胞联盟。在视野领域，这种协作方法在阐明与年龄相关的黄斑变性相关的遗传易感性方面取得了巨大的成功。
We discuss the use of pluripotent stem cell lines carrying fluorescent reporters driven by retinal promoters to derive three-dimensional (3-D) retina in culture and how this system can be exploited for elucidating human retinal biology, creating disease models in a dish, and designing targeted drug screens for retinal and macular degeneration. Furthermore, we realize that stem cell investigations are labor-intensive and require extensive resources. To expedite scientific discovery by sharing of resources and to avoid duplication of efforts, we propose the formation of a Retinal Stem Cell Consortium. In the field of vision, such collaborative approaches have been enormously successful in elucidating genetic susceptibility associated with age-related macular degeneration.