USE OF RESTRICTION FRAGMENT LENGTH POLYMORPHISMS TO DETERMINE THE CLONAL ORIGIN OF HUMAN-TUMORS
USE OF RESTRICTION FRAGMENT LENGTH POLYMORPHISMS TO DETERMINE THE CLONAL ORIGIN OF HUMAN-TUMORS
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DOI：
10.1126/science.2982210
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发表时间：
1985-01-01
期刊：
影响因子：
56.9
通讯作者：
FEINBERG, AP
中科院分区：
文献类型：
--
作者：
VOGELSTEIN, B;FEARON, ER;FEINBERG, AP
设计了一种新的策略来确定人类肿瘤的克隆起源。该策略涉及使用克隆的多态性X染色体基因和2种限制性内切酶。第一种核酸内切酶通过限制性片段长度的DNA多态性来区分基因的父系和母系拷贝。第二种核酸内切酶通过DNA甲基化的变化来区分该基因的活性拷贝和非活性拷贝。作为该策略的例证，3种人类癌症均显示为单克隆的。所描述的分析应具有广泛的临床和实验应用。
A novel strategy to determine the clonal origin of human tumors was devised. The strategy involves the use of a cloned polymorphic X-chromosomal gene and 2 restriction endonucleases. The 1st endonuclease distinguishes the paternal and maternal copies of the gene through a DNA polymorphism of restriction fragment length. The 2nd endonuclease distinguished active from inactive copies of this gene through changes in DNA methylation. As illustrations of this strategy, 3 human cancers were each shown to be monoclonal. The analaysis described should have a wide variety of clinical and experimental applications.