Erythrocyte membrane coated nanoparticle-based control releasing hydrogen sulfide system protects ischemic myocardium
Erythrocyte membrane coated nanoparticle-based control releasing hydrogen sulfide system protects ischemic myocardium
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DOI：
10.2217/nnm-2020-0404
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发表时间：
2021-02-18
期刊：
影响因子：
5.5
通讯作者：
Sun, Xiaotian
中科院分区：
文献类型：
--
作者：
Huang, Kai;Wen, Shuyan;Sun, Xiaotian
目的：构建长循环缓释H2S系统，探讨其对心肌缺血再灌注（I/R）损伤的保护作用。材料与方法：制备了包被红细胞（RBC）膜、携带三硫二烯丙基（DATS）的介孔氧化铁纳米颗粒（RBC-DATS-MIONs）并对其进行了表征。体外研究细胞毒性和细胞摄取，然后在体内评估I/R损伤后的安全性、分布和对心功能的影响。结果：RBC-DATS-MIONs具有良好的生物相容性，延长循环时间，可在血浆和心肌中控制H2S的释放。它们对体外缺氧/再氧化模型和体内心肌I/R模型均有良好的治疗作用，其作用机制包括抗凋亡、抗炎和抗氧化等。结论：通过延长H2S的释放过程，为充分利用H2S的保护作用提供了一个新的潜在平台。
Aim: To construct a long circulatory and sustained releasing H2S system and explore its protective effects on myocardial ischemia and reperfusion (I/R) injury. Materials & methods: Red blood cell (RBC) membrane-coated, diallyl trisulfide (DATS)-carrying mesoporous iron oxide nanoparticles (MIONs) (RBC-DATS-MIONs) were prepared and characterized. Cytotoxicity and cellular uptake were studied in vitro, followed by in vivo assessment of safety, distribution and effect on cardiac function following I/R injury. Results: RBC-DATS-MIONs exhibited excellent biocompatibility, extended circulatory time and controlled-release of H2S in plasma and myocardium. They exhibited superior therapeutic effects on in vitro hypoxia/reoxygenation models and in vivo myocardial I/R models, which involved various mechanisms, including anti-apoptosis, anti-inflammatory and antioxidant activities. Conclusion: This work provides a new potential platform for best utilizing the protective effects of H2S by prolonging its releasing process.