Beta-glucan-CRM197 conjugates as candidates antifungal vaccines
Beta-glucan-CRM197 conjugates as candidates antifungal vaccines
复制标题
DOI：
10.1016/j.vaccine.2010.01.012
复制
发表时间：
2010-03-19
期刊：
影响因子：
5.5
通讯作者：
Cassone, Antonio
中科院分区：
文献类型：
--
作者：
Bromuro, Carla;Romano, Maria;Cassone, Antonio
昆布多糖-白喉类毒素（CRM 197）缀合物疫苗在小鼠中赋予针对真菌感染的保护。我们现在已经产生了新型β-葡聚糖-CRM 197疫苗，其具有天然（Curd-CRM 197）或合成的线性（15 mer-CRM 197）或β-（1，6）-支链（17 mer-CRM 197）β-（1，3）-寡糖，与人类可接受的佐剂MF 59一起配制。凝乳-CRM 197和15聚体-CRM 197结合物诱导高滴度的抗β-（1，3）-葡聚糖IgG，但不诱导抗β-（1，6）-葡聚糖的抗体，对用C.白色念珠菌相比之下，除了抗β-（1，3）-葡聚糖IgG之外还诱导抗β-（1，6）-葡聚糖抗体的17聚体-CRM 197缀合物是非保护性的。这些数据为β-葡聚糖表位介导抗真菌保护提供了一些见解，并为开发针对真菌疾病的合成寡糖疫苗开辟了道路。(C)2010爱思唯尔有限公司版权所有。
A laminarin-diphtheria toxoid (CRM197) conjugate vaccine conferred protection against fungal infections in mice. We have now generated novel beta-glucan-CRM197 vaccines, with either natural (Curd-CRM197) or synthetic linear (15mer-CRM197), or beta-(1,6)-branched (17mer-CRM197) beta-(1,3)-oligosaccharides, formulated with the human-acceptable adjuvant MF59. Curd-CRM197 and 15mer-CRM197 conjugates, which induced high titers of anti-beta-(1,3)-glucan IgG, but no antibodies against beta-(1,6)-glucan, conferred protection to mice lethally challenged with C. albicans. In contrast, the 17mer-CRM197 conjugate, which induced anti-beta-(1,6)-glucan antibodies in addition to the anti-beta-(1,3)-glucan IgG, was non-protective. These data provide some insights on beta-glucan epitope(s) mediating antifungal protection and open the way to develop a synthetic oligosaccharide vaccine against fungal diseases. (C) 2010 Elsevier Ltd. All rights reserved.