Molecular Markers Reveal Infestation Dynamics of the Bed Bug (Hemiptera: Cimicidae) Within Apartment Buildings
Molecular Markers Reveal Infestation Dynamics of the Bed Bug (Hemiptera: Cimicidae) Within Apartment Buildings
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DOI：
10.1603/me11256
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发表时间：
2012-05-01
影响因子：
2.1
通讯作者：
Vargo, Edward L.
中科院分区：
文献类型：
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作者：
Booth, Warren;Saenz, Virna L.;Vargo, Edward L.
臭虫 Cimex lectularius L.（半翅目：臭虫科）近年来在全球范围内经历了非同寻常的复苏，但其原因仍知之甚少。臭虫曾经被认为是低社会经济阶层的害虫，现在在所有住宅环境中广泛发现，在多公寓建筑中广泛存在。在此类建筑物内，了解种群遗传结构和扩散模式可能对于制定有效的控制策略至关重要。在这里，我们描述了通过下一代 454 焦磷酸测序技术开发的 24 个高分辨率微卫星标记，以及它们在阐明美国三座多层公寓楼内的侵染动态方面的应用。结果揭示了可能代表地理或地点差异的对比特征。在北卡罗来纳州罗利市，一栋公寓楼内的感染似乎是从一次传入开始，随后迅速蔓延。在新泽西州泽西城，两栋建筑中明显可见两种或多种引入，随后传播。所有建筑物的单间公寓内的种群都具有高相关性和低多样性的特点，这表明其基础是小型的、基因贫乏的繁殖体。无论独特引入的数量有多少，遗传数据表明，建筑物内的传播是广泛的，支持跨越多个楼层的相邻房间或公寓内部和之间的主动传播和人为介导的传播。
The bed bug, Cimex lectularius L. (Hemiptera: Cimicidae), has experienced an extraordinary global resurgence in recent years, the reasons for which remain poorly understood. Once considered a pest of lower socioeconomic classes, bed bugs are now found extensively across all residential settings, with widespread infestations established in multiapartment buildings. Within such buildings, understanding the population genetic structure and patterns of dispersal may prove critical to the development of effective control strategies. Here, we describe the development of 24 high-resolution microsatellite markers through next generation 454 pyrosequencing and their application to elucidate infestation dynamics within three multistory apartment buildings in the United States. Results reveal contrasting characteristics potentially representative of geographic or locale differences. In Raleigh, NC, an infestation within an apartment building seemed to have started from a single introduction followed by extensive spread. In Jersey City, NJ, two or more introductions followed by spread are evident in two buildings. Populations within single apartments in all buildings were characterized by high levels of relatedness and low levels of diversity, indicative of foundation from small, genetically depauperate propagules. Regardless of the number of unique introductions, genetic data indicate that spread within buildings is extensive, supporting both active and human-mediated dispersal within and between adjacent rooms or apartments spanning multiple floors.