The destruction of pulmonary and hepatic cytochrome P-450 by phthaladehyde.
The destruction of pulmonary and hepatic cytochrome P-450 by phthaladehyde.
复制标题
邻苯二甲醛对肺和肝细胞色素 P-450 的破坏。
DOI：
10.1016/0041-008x(79)90040-1
复制
发表时间：
1979
影响因子：
3.8
通讯作者：
J. Patel
中科院分区：
文献类型：
--
作者：
J. Patel
向肺和肝微粒体中添加邻苯二甲醛导致所有检测浓度下细胞色素P-450的破坏。肺和肝细胞色素P-450的邻苯二甲醛破坏不需要NADPH。此外，邻苯二甲醛的肺和肝微粒体悬浮液也导致对二甲苯羟化酶和苄非他明N-脱甲基酶活性的抑制。在体外对二甲苯羟化酶和苄非他明N-脱甲基酶的抑制，以及P-450的破坏，可以通过补充微粒体与肝细胞质或纯化的醛脱氢酶。当邻苯二甲醛加入到肺或肝微粒体中时，没有发生脂质过氧化反应。提示邻苯二甲醛可用于研究这些组织制剂中的体外药物代谢反应。
Addition of phthalaldehyde to pulmonary and hepatic microsomes resulted in destruction of cytochrome P-450 at all concentrations of phthalaldehyde tested. Phthalaldehyde destruction of pulmonary and hepatic cytochrome P-450 did not require NADPH. Addition of phthalaldehyde to pulmonary and hepatic microsomal suspension also resulted in inhibition of p-xylene hydroxylase and benzphetamine N-demethylase activities. The in vitro inhibition of p-xylene hydroxylase and benzphetamine N-demethylase as well as the destruction of P-450 could be prevented by supplementing microsomes with hepatic cytosol or purified aldehyde dehydrogenase. Lipid peroxidation did not occur when phthalaldehyde was added to either pulmonary or hepatic microsomes. It is suggested that phthalaldehyde can be used to study the in vitro drug metabolism reactions in these tissue preparations.