A "traffic control" role for TGFbeta3: orchestrating dermal and epidermal cell motility during wound healing.
A "traffic control" role for TGFbeta3: orchestrating dermal and epidermal cell motility during wound healing.
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DOI：
10.1083/jcb.200507111
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发表时间：
2006-03-27
影响因子：
7.8
通讯作者：
Li, Wei
中科院分区：
文献类型：
--
作者：
Bandyopadhyay, Balaji;Fan, Jianhua;Guan, Shengxi;Li, Yong;Chen, Mei;Woodley, David T;Li, Wei
细胞迁移是皮肤创面愈合的限速事件。在未受伤的皮肤中，细胞由血浆滋养。当皮肤受伤时，常驻细胞第一次遇到血清。随着伤口的愈合，细胞经历了血清向血浆的转变。在这项研究中，我们报告了人血清选择性地促进表皮细胞迁移和阻止真皮细胞迁移。相比之下，人的血浆促进了真皮细胞的迁移，而不是表皮细胞的迁移。这种开关是由转化生长因子β3水平和转化生长因子β受体(TβR)II型水平控制的，前者在血浆中检测不到，在血清中高，后者在表皮细胞中低，在真皮细胞中高。从血清中去除转化生长因子β3可将血清转化为类似血浆的试剂。向血浆中加入转化生长因子β3可将其转化为血清样试剂。真皮细胞中TβRII的下调或表皮细胞中TβRII的上调分别逆转了它们对血清和血浆的迁移反应。因此，创面愈合过程中自然产生的血浆→血清→血浆转变协调真皮和表皮细胞的有序迁移。
Cell migration is a rate-limiting event in skin wound healing. In unwounded skin, cells are nourished by plasma. When skin is wounded, resident cells encounter serum for the first time. As the wound heals, the cells experience a transition of serum back to plasma. In this study, we report that human serum selectively promotes epidermal cell migration and halts dermal cell migration. In contrast, human plasma promotes dermal but not epidermal cell migration. The on-and-off switch is operated by transforming growth factor (TGF) β3 levels, which are undetectable in plasma and high in serum, and by TGFβ receptor (TβR) type II levels, which are low in epidermal cells and high in dermal cells. Depletion of TGFβ3 from serum converts serum to a plasmalike reagent. The addition of TGFβ3 to plasma converts it to a serumlike reagent. Down-regulation of TβRII in dermal cells or up-regulation of TβRII in epidermal cells reverses their migratory responses to serum and plasma, respectively. Therefore, the naturally occurring plasma→serum→plasma transition during wound healing orchestrates the orderly migration of dermal and epidermal cells.