Identification of key tissue type for antler regeneration through pedicle periosteum deletion
Identification of key tissue type for antler regeneration through pedicle periosteum deletion
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DOI：
10.1007/s00441-006-0333-y
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发表时间：
2007-04-01
影响因子：
3.6
通讯作者：
Clark, Dawn E.
中科院分区：
文献类型：
--
作者：
Li, Chunyi;Mackintosh, Colin G.;Clark, Dawn E.
表型再生是再生医学的“圣杯”。如果要建立对支持这一过程的因素的基本了解，那么研究旨在研究表型再生的各种模式是必不可少的。鹿角是唯一一种每年经历表型再生周期的哺乳动物的附肢。在我们以前的研究中，我们已经报道鹿角再生的组织发生依赖于驻留在椎弓根骨膜(PP)内的细胞。本研究通过涉及PP缺失的功能研究阐述了这一发现。选取4岁公鹿和4只2龄公鹿进行PP完全缺失和PP部分缺失实验。在全部PP缺失组的动物中，有一只在整个鹿茸生长季节没有表现出鹿角再生的迹象。与相应的假手术侧相比，两侧鹿角再生明显延迟，一侧鹿角再生轻微延迟(分别为34天、20天和7天)。组织学检查表明，延迟鹿角来源于再生的PP。出乎意料的是，从全骨膜去掉的椎弓根再生鹿角的能力取决于手术时鹿角的长度。在4只部分PP缺失的鹿中，2只在没有椎弓根本身参与的情况下，仅从椎弓根上残留的PP再生鹿角。PP缺失实验的综合结果令人信服地证明PP的细胞负责鹿角的再生。
Epimorphic regeneration is the "holy grail" of regenerative medicine. Research aimed at investigating the various models of epimorphic regeneration is essential if a fundamental understanding of the factors underpinning this process are to be established. Deer antlers are the only mammalian appendages that are subject to an annual cycle of epimorphic regeneration. In our previous studies, we have reported that histogenesis of antler regeneration relies on cells resident within the pedicle periosteum (PP). The present study elaborates this finding by means of functional studies involving the deletion of PP. Four yearling and four 2-year-old stags were selected for total PP deletion or partial PP deletion experiments. Of the animals in the total PP deletion group, one showed no signs of antler regeneration throughout the antler growth season. Two showed substantial and one showed marginal delays in antler regeneration (at 34, 20 and 7 days, respectively) compared with the corresponding sham-operated sides. Histological investigation revealed that the delayed antlers were derived from regenerated PP. Unexpectedly, the regenerative capacity of the antler from the total periosteum-deleted pedicles depended on antler length at surgery. Of the four deer that had partial PP deletion, two regenerated antlers exclusively from the left-over PP on the pedicle shafts in the absence of participation from the pedicle bone proper. The combined results from the PP deletion experiments convincingly demonstrate that the cells of the PP are responsible for antler regeneration.